ArticleProceedings of the National Academy of Sciences of the United States of America2013
Chemical polysialylation of human recombinant butyrylcholinesterase delivers a long-acting bioscavenger for nerve agents in vivo.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Chemical neighborhood exploration for substrate discovery in biocatalysis.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Design of a novel long-acting insulin analogs by acetylation modification and compared with insulin Icodec.Scientific reports · 2025Article
- Upconversion Nanoparticles Decorated with Polysialic Acid for Solid Tumors Visualization In Vivo.Doklady. Biochemistry and biophysics · 2021Article
- Counteracting poisoning with chemical warfare nerve agents.Arhiv za higijenu rada i toksikologiju · 2020Review
- Engineering orthogonal human O-linked glycoprotein biosynthesis in bacteria.Nature chemical biology · 2020Article
- Multiscale computation delivers organophosphorus reactivity and stereoselectivity to immunoglobulin scavengers.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Acetylcholinesterase: The "Hub" for Neurodegenerative Diseases and Chemical Weapons Convention.Biomolecules · 2020Review
- Enhancing the Butyrylcholinesterase Activity in HEK-293 Cell Line by Dual-Promoter Vector Decorated on Lipofectamine.Drug design, development and therapy · 2020Article
- Improving designer glycan production inMetabolic engineering communications · 2019Article
- Cryo-EM structure of the native butyrylcholinesterase tetramer reveals a dimer of dimers stabilized by a superhelical assembly.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Purification of recombinant human butyrylcholinesterase on Hupresin®.Journal of chromatography. B, Analytical technologies in the biomedical and life sciences · 2018Article
- Cholinesterases and the fine line between poison and remedy.Biochemical pharmacology · 2018Review
- QM/MM Description of Newly Selected Catalytic Bioscavengers Against Organophosphorus Compounds Revealed Reactivation Stimulus Mediated by Histidine Residue in the Acyl-Binding Loop.Frontiers in pharmacology · 2018Article
- Microfluidic droplet platform for ultrahigh-throughput single-cell screening of biodiversity.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Organophosphate-Hydrolyzing Enzymes as First-Line of Defence Against Nerve Agent-Poisoning: Perspectives and the Road Ahead.The protein journal · 2016Review
- Engineering of complex protein sialylation in plants.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Semicontinuous Bioreactor Production of Recombinant Butyrylcholinesterase in Transgenic Rice Cell Suspension Cultures.Frontiers in plant science · 2016Article
- Excessive Labeling Technique Provides a Highly Sensitive Fluorescent Probe for Real-time Monitoring of Biodegradation of Biopolymer Pharmaceuticals in vivo.Acta naturae · 2014Article
- Oligomerization status influences subcellular deposition and glycosylation of recombinant butyrylcholinesterase in Nicotiana benthamiana.Plant biotechnology journal · 2014Article
- Expression of human butyrylcholinesterase with an engineered glycosylation profile resembling the plasma-derived orthologue.Biotechnology journal · 2014Article
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Authors and funding
17 authors.
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Abstract
The creation of effective bioscavengers as a pretreatment for exposure to nerve agents is a challenging medical objective. We report a recombinant method using chemical polysialylation to generate bioscavengers stable in the bloodstream. Development of a CHO-based expression system using genes encoding human butyrylcholinesterase and a proline-rich peptide under elongation factor promoter control resulted in self-assembling, active enzyme multimers. Polysialylation gives bioscavengers with enhanced pharmacokinetics which protect mice against 4.2 LD(50) of S-(2-(diethylamino)ethyl) O-isobutyl methanephosphonothioate without perturbation of long-term behavior.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.