ArticleMethods in molecular biology (Clifton, N.J.)2013
Biomarkers of cell senescence assessed by imaging cytometry.
Article in Methods in molecular biology (Clifton, N.J.), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
40 citing papers in PubMed, 64 citations in OpenAlex.
- Oxidative Stress, Inflammation, and Cellular Senescence in Neuropathic Pain: Mechanistic Crosstalk.Antioxidants (Basel, Switzerland) · 2025Review
- Efficient and simple protocol for mechanical isolation of foreskin-derived primary human dermal fibroblasts for replicative senescence studies.BMC research notes · 2025Article
- Senescence-Associated Chromatin Rewiring Promotes Inflammation and Transposable Element Activation.bioRxiv : the preprint server for biology · 2025Article
- The chemical chaperone 4-phenylbutyric acid rescues molecular cell defects of COL3A1 mutations that cause vascular Ehlers Danlos Syndrome.Cell death discovery · 2025Article
- Petroleum ether extract of3 Biotech · 2025Article
- Immortalized mammosphere-derived epithelial cells retain a bioactive secretome with antimicrobial, regenerative, and immunomodulatory properties.Stem cell research & therapy · 2024Article
- Chemical genetic screens reveal defective lysosomal trafficking as synthetic lethal with NF1 loss.Journal of cell science · 2024Article
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- Article
- Protective Effect of Curcumin on D-Galactose-Induced Senescence and Oxidative Stress in LLC-PK1 and HK-2 Cells.Antioxidants (Basel, Switzerland) · 2024Article
- From pixels to phenotypes: Integrating image-based profiling with cell health data as BioMorph features improves interpretability.Molecular biology of the cell · 2024Article
- Emerging role of cellular senescence in normal lung development and perinatal lung injury.Chinese medical journal pulmonary and critical care medicine · 2024Article
- Cellular Senescence Program is Sensitive to Physical Differences in Polymeric Tissue Scaffolds.ACS materials Au · 2024Article
- Autophagy and senescence facilitate the development of antiestrogen resistance in ER positive breast cancer.Frontiers in endocrinology · 2024Review
- REV7: a small but mighty regulator of genome maintenance and cancer development.Frontiers in oncology · 2024Review
- A natural variation-based screen in mouse cells reveals USF2 as a regulator of the DNA damage response and cellular senescence.G3 (Bethesda, Md.) · 2023Article
- Article
- Hallmarks and Biomarkers of Skin Senescence: An Updated Review of Skin Senotherapeutics.Antioxidants (Basel, Switzerland) · 2023Review
- Toxicity Impacts on Human Adipose Mesenchymal Stem/Stromal Cells Acutely Exposed to Aroclor and Non-Aroclor Mixtures of Polychlorinated Biphenyl.Environmental science & technology · 2023Article
- Lessons in aging from Myc knockout mouse models.Frontiers in cell and developmental biology · 2023Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The characteristic features of senescent cells such as their "flattened" appearance, enlarged nuclei and low saturation density at the plateau phase of cell growth, can be conveniently measured by image-assisted cytometry such as provided by a laser scanning cytometer (LSC). The "flattening" of senescent cells is reflected by a decline in local density of DNA-associated staining with 4,6-diamidino-2-phenylindole (DAPI) and is paralleled by an increase in nuclear area. Thus, the ratio of the maximal intensity of DAPI fluorescence per nucleus to the nuclear area provides a very sensitive morphometric biomarker of "depth" of senescence, which progressively declines during senescence induction. Also recorded is cellular DNA content revealing cell cycle phase, as well as the saturation cell density at plateau phase of growth, which is dramatically decreased in cultures of senescent cells. Concurrent immunocytochemical analysis of expression of p21(WAF1), p16(INK4a), or p27(KIP1) cyclin kinase inhibitors provides additional markers of senescence. These biomarker indices can be expressed in quantitative terms ("senescence indices") as a fraction of the same markers of the exponentially growing cells in control cultures.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.