ArticleSteroids2013
Membrane-initiated estradiol signaling in immortalized hypothalamic N-38 neurons.
Article in Steroids, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 27 citations in OpenAlex.
- Review
- Localisation of oestrogen receptors in stem cells and in stem cell-derived neurons of the mouse.Journal of neuroendocrinology · 2023Article
- Investigation of cellular uptake mechanism of functionalised gold nanoparticles into breast cancer using SERS.Chemical science · 2020Article
- ERαΔ4, an ERα splice variant missing exon4, interacts with caveolin-3 and mGluR2/3.Journal of neuroendocrinology · 2019Article
- Extranuclear signaling by ovarian steroids in the regulation of sexual receptivity.Hormones and behavior · 2018Review
- Estrogen and Progesterone Integration in an in vitro Model of RP3V Kisspeptin Neurons.Neuroendocrinology · 2018Article
- Estradiol Membrane-Initiated Signaling in the Brain Mediates Reproduction.Trends in neurosciences · 2017Review
- Integrating Neural Circuits Controlling Female Sexual Behavior.Frontiers in systems neuroscience · 2017Review
- Actions of Steroids: New Neurotransmitters.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2016Review
- Estradiol Membrane-Initiated Signaling and Female Reproduction.Comprehensive Physiology · 2015Review
- Article
- Membrane-initiated non-genomic signaling by estrogens in the hypothalamus: cross-talk with glucocorticoids with implications for behavior.Frontiers in endocrinology · 2015Review
- Estradiol modulates translocator protein (TSPO) and steroid acute regulatory protein (StAR) via protein kinase A (PKA) signaling in hypothalamic astrocytes.Endocrinology · 2014Article
- Temporal and concentration-dependent effects of oestradiol on neural pathways mediating sexual receptivity.Journal of neuroendocrinology · 2013Review
- Article
- Fluorescently-Labeled Estradiol Internalization and Membrane Trafficking in Live N-38 Neuronal Cells Visualized with Total Internal Reflection Fluorescence Microscopy.Journal of steroids & hormonal science · 2013Article
- Membrane-initiated estradiol actions mediate structural plasticity and reproduction.Frontiers in neuroendocrinology · 2012Review
- Estradiol signaling in the regulation of reproduction and energy balance.Frontiers in neuroendocrinology · 2012Review
- Neurosteroids, trigger of the LH surge.The Journal of steroid biochemistry and molecular biology · 2012Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Regulation of sexual reproduction by estradiol involves the activation of estrogen receptors (ERs) in the hypothalamus. Of the two classical ERs involved in reproduction, ERα appears to be the critical isoform. The role of ERα in reproduction has been found to involve a nuclear ERα that induces a genomic mechanism of action. More recently, a plasma membrane ERα has been shown to trigger signaling pathways involved in reproduction. Mechanisms underlying membrane-initiated estradiol signaling are emerging, including evidence that activation of plasma membrane ERα involves receptor trafficking. The present study examined the insertion of ERα into the plasma membrane of N-38 neurons, an immortalized murine hypothalamic cell line. We identified, using western blotting and PCR that N-38 neurons express full-length 66kDa ERα and a 52kDa ERα spliced variant missing the fourth exon - ERαΔ4. Using surface biotinylation, we observed that treatment of N-38 neurons with estradiol or with a membrane impermeant estradiol elevated plasma membrane ERα protein levels, indicating that membrane signaling increased receptor insertion into the cell membrane. Insertion of ERα was blocked by the ER antagonist ICI 182,780 or with the protein kinase C (PKC) pathway inhibitor bisindolylmaleimide (BIS). Downstream membrane-initiated signaling was confirmed by estradiol activation of PKC-theta (PKCθ) and the release of intracellular calcium. These results indicate that membrane ERα levels in N-38 neurons are dynamically autoregulated by estradiol.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.