ArticleJournal of visualized experiments : JoVE2012
Neural Explant Cultures from Xenopus laevis.
Article in Journal of visualized experiments : JoVE, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 23 citations in OpenAlex.
- The TOG5 domain of CKAP5 is required to interact with F-actin and promote microtubule advancement in neurons.Molecular biology of the cell · 2024Article
- CKAP5 enables formation of persistent actin bundles templated by dynamically instable microtubules.Current biology : CB · 2024Article
- A Methodology for the Enzymatic Isolation of Embryonic Hypothalamus Tissue and Its Acute or Post-Culture Analysis by Multiplex Hybridisation Chain Reaction.Bio-protocol · 2023Article
- Tau, XMAP215/Msps and Eb1 co-operate interdependently to regulate microtubule polymerisation and bundle formation in axons.PLoS genetics · 2021Article
- Live Imaging of Cytoskeletal Dynamics in EmbryonicCold Spring Harbor protocols · 2021Article
- Functional assessment of the "two-hit" model for neurodevelopmental defects in Drosophila and X. laevis.PLoS genetics · 2021Article
- Xenopus leads the way: Frogs as a pioneering model to understand the human brain.Genesis (New York, N.Y. : 2000) · 2021Review
- Caveolin 1 is required for axonal outgrowth of motor neurons and affects Xenopus neuromuscular development.Scientific reports · 2020Article
- Investigating the impact of the phosphorylation status of tyrosine residues within the TACC domain of TACC3 on microtubule behavior during axon growth and guidance.Cytoskeleton (Hoboken, N.J.) · 2020Article
- NCBP2 modulates neurodevelopmental defects of the 3q29 deletion in Drosophila and Xenopus laevis models.PLoS genetics · 2020Article
- XMAP215 promotes microtubule-F-actin interactions to regulate growth cone microtubules during axon guidance inJournal of cell science · 2019Article
- Characterization of Xenopus laevis guanine deaminase reveals new insights for its expression and function in the embryonic kidney.Developmental dynamics : an official publication of the American Association of Anatomists · 2019Article
- The microtubule plus-end-tracking protein TACC3 promotes persistent axon outgrowth and mediates responses to axon guidance signals during development.Neural development · 2017Article
- Xenopus laevis as a model system to study cytoskeletal dynamics during axon pathfinding.Genesis (New York, N.Y. : 2000) · 2017Review
- Exploring the developmental mechanisms underlying Wolf-Hirschhorn Syndrome: Evidence for defects in neural crest cell migration.Developmental biology · 2016Review
- Conserved roles for cytoskeletal components in determining laterality.Integrative biology : quantitative biosciences from nano to macro · 2016Review
- Using Xenopus laevis retinal and spinal neurons to study mechanisms of axon guidance in vivo and in vitro.Seminars in cell & developmental biology · 2016Review
- Xenopus TACC1 is a microtubule plus-end tracking protein that can regulate microtubule dynamics during embryonic development.Cytoskeleton (Hoboken, N.J.) · 2015Article
- Using plusTipTracker software to measure microtubule dynamics in Xenopus laevis growth cones.Journal of visualized experiments : JoVE · 2014Article
- Growth cone-specific functions of XMAP215 in restricting microtubule dynamics and promoting axonal outgrowth.Neural development · 2013Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The complex process of axon guidance is largely driven by the growth cone, which is the dynamic motile structure at the tip of the growing axon. During axon outgrowth, the growth cone must integrate multiple sources of guidance cue information to modulate its cytoskeleton in order to propel the growth cone forward and accurately navigate to find its specific targets(1). How this integration occurs at the cytoskeletal level is still emerging, and examination of cytoskeletal protein and effector dynamics within the growth cone can allow the elucidation of these mechanisms. Xenopus laevis growth cones are large enough (10-30 microns in diameter) to perform high-resolution live imaging of cytoskeletal dynamics (e.g.(2-4) ) and are easy to isolate and manipulate in a lab setting compared to other vertebrates. The frog is a classic model system for developmental neurobiology studies, and important early insights into growth cone microtubule dynamics were initially found using this system(5-7) . In this method(8), eggs are collected and fertilized in vitro, injected with RNA encoding fluorescently tagged cytoskeletal fusion proteins or other constructs to manipulate gene expression, and then allowed to develop to the neural tube stage. Neural tubes are isolated by dissection and then are cultured, and growth cones on outgrowing neurites are imaged. In this article, we describe how to perform this method, the goal of which is to culture Xenopus laevis growth cones for subsequent high-resolution image analysis. While we provide the example of +TIP fusion protein EB1-GFP, this method can be applied to any number of proteins to elucidate their behaviors within the growth cone.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.