ArticleStem cells and development2013
Plasticity of calcium signaling cascades in human embryonic stem cell-derived neural precursors.
Article in Stem cells and development, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 33 citations in OpenAlex.
- Temporal Interference Stimulation Enhances Neural Regeneration.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Metal Ion Signaling in Biomedicine.Chemical reviews · 2025Review
- Isogenic iPSC-derivedFrontiers in neuroscience · 2025Article
- Regulation of store-operated CaeLife · 2023Article
- Different iPSC-derived neural stem cells shows various spectrums of spontaneous differentiation during long term cultivation.Frontiers in molecular neuroscience · 2023Article
- IPSC-Derived Human Neurons with GCaMP6s Expression Allow In Vitro Study of Neurophysiological Responses to Neurochemicals.Neurochemical research · 2022Article
- The calcium dynamics of human dental pulp stem cells stimulated with tricalcium silicate-based cements determine their differentiation and mineralization outcome.Scientific reports · 2021Article
- Transplantation of Neural Precursors Derived from Induced Pluripotent Cells Preserve Perineuronal Nets and Stimulate Neural Plasticity in ALS Rats.International journal of molecular sciences · 2020Article
- StableFrontiers in molecular neuroscience · 2018Article
- Functional expression of the CaActa pharmacologica Sinica · 2017Article
- Purinergic Receptor Expression and Potential Association with Human Embryonic Stem Cell-Derived Oligodendrocyte Progenitor Cell Development.Cell journal · 2017Article
- Neuronal P2X7 Receptor: Involvement in Neuronal Physiology and Pathology.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2017Review
- Neuroprotective Potential of Cell-Based Therapies in ALS: From Bench to Bedside.Frontiers in neuroscience · 2017Review
- One-Step Seeding of Neural Stem Cells with Vitronectin-Supplemented Medium for High-Throughput Screening Assays.Journal of biomolecular screening · 2016Article
- Physiology of spontaneous [Ca(2+)]i oscillations in the isolated vasopressin and oxytocin neurones of the rat supraoptic nucleus.Cell calcium · 2016Article
- Getting it right before transplantation: example of a stem cell model with regenerative potential for the CNS.Frontiers in cell and developmental biology · 2014Article
- Asymmetric cell division of stem cells in the lung and other systems.Frontiers in cell and developmental biology · 2014Review
- Bovine Brain: An in vitro Translational Model in Developmental Neuroscience and Neurodegenerative Research.Frontiers in pediatrics · 2014Review
- Plasticity of calcium signaling cascades in human embryonic stem cell-derived neural precursors.Stem cells and development · 2013Article
Corrections and comments
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Authors and funding
5 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human embryonic stem cell-derived neural precursors (hESC NPs) are considered to be a promising tool for cell-based therapy in central nervous system injuries and neurodegenerative diseases. The Ca(2+) ion is an important intracellular messenger essential for the regulation of various cellular functions. We investigated the role and physiology of Ca(2+) signaling to characterize the functional properties of CCTL14 hESC NPs during long-term maintenance in culture (in vitro). We analyzed changes in cytoplasmic Ca(2+) concentration ([Ca(2+)]i) evoked by high K(+), adenosine-5'-triphosphate (ATP), glutamate, γ-aminobutyric acid (GABA), and caffeine in correlation with the expression of various neuronal markers in different passages (P6 through P10) during the course of hESC differentiation. We found that only differentiated NPs from P7 exhibited significant and specific [Ca(2+)]i responses to various stimuli. About 31% of neuronal-like P7 NPs exhibited spontaneous [Ca(2+)]i oscillations. Pharmacological and immunocytochemical assays revealed that P7 NPs express L- and P/Q-type Ca(2+) channels, P2X2, P2X3, P2X7, and P2Y purinoreceptors, glutamate receptors, and ryanodine (RyR1 and RyR3) receptors. The ATP- and glutamate-induced [Ca(2+)]i responses were concentration-dependent. Higher glutamate concentrations (over 100 μM) caused cell death. Responses to ATP were observed in the presence or in the absence of extracellular Ca(2+). These results emphasize the notion that with time in culture, these cells attain a transient period of operative Ca(2+) signaling that is predictive of their ability to act as stem elements.
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