ArticleFrontiers in neural circuits2012
Brain selective transgene expression in zebrafish using an NRSE derived motif.
Article in Frontiers in neural circuits, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 34 citations in OpenAlex.
- Negative-Valence Neurons in the Larval Zebrafish Pallium.bioRxiv : the preprint server for biology · 2026Article
- Pth4 neurons define a novel hypothalamic circuit that promotes sleep via brainstem monoaminergic neurons.Current biology : CB · 2026Article
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- Pth4 neurons define a novel hypothalamic circuit that promotes sleep via brainstem monoaminergic neurons.bioRxiv : the preprint server for biology · 2025Article
- Transsynaptic labeling and transcriptional control of zebrafish neural circuits.Nature neuroscience · 2025Article
- Radial astrocyte synchronization modulates the visual system during behavioral-state transitions.Neuron · 2023Article
- Thalamic regulation of a visual critical period and motor behavior.Cell reports · 2023Article
- A Critical Review of Zebrafish Neurological Disease Models-2. Application: Functional and Neuroanatomical Phenotyping Strategies and Chemical Screens.Oxford open neuroscience · 2023Article
- A Critical Review of Zebrafish Neurological Disease Models-1. The Premise: Neuroanatomical, Cellular and Genetic Homology and Experimental Tractability.Oxford open neuroscience · 2023Article
- A cerebellar-prepontine circuit for tonic immobility triggered by an inescapable threat.Science advances · 2022Article
- Prepontine non-giant neurons drive flexible escape behavior in zebrafish.PLoS biology · 2019Article
- Brain-wide cellular resolution imaging of Cre transgenic zebrafish lines for functional circuit-mapping.eLife · 2019Article
- Opportunities and challenges for using the zebrafish to study neuronal connectivity as an endpoint of developmental neurotoxicity.Neurotoxicology · 2018Review
- Article
- Genetic targeting and anatomical registration of neuronal populations in the zebrafish brain with a new set of BAC transgenic tools.Scientific reports · 2017Article
- QRFP and Its Receptors Regulate Locomotor Activity and Sleep in Zebrafish.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2016Article
- Genetic Ablation, Sensitization, and Isolation of Neurons Using Nitroreductase and Tetrodotoxin-Insensitive Channels.Methods in molecular biology (Clifton, N.J.) · 2016Article
- Gsx1 expression defines neurons required for prepulse inhibition.Molecular psychiatry · 2015Article
- Increased functional protein expression using nucleotide sequence features enriched in highly expressed genes in zebrafish.Nucleic acids research · 2015Article
- A 3D Searchable Database of Transgenic Zebrafish Gal4 and Cre Lines for Functional Neuroanatomy Studies.Frontiers in neural circuits · 2015Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
Transgenic technologies enable the manipulation and observation of circuits controlling behavior by permitting expression of genetically encoded reporter genes in neurons. Frequently though, neuronal expression is accompanied by transgene expression in non-neuronal tissues, which may preclude key experimental manipulations, including assessment of the contribution of neurons to behavior by ablation. To better restrict transgene expression to the nervous system in zebrafish larvae, we have used DNA sequences derived from the neuron-restrictive silencing element (NRSE). We find that one such sequence, REx2, when used in conjunction with several basal promoters, robustly suppresses transgene expression in non-neuronal tissues. Both in transient transgenic experiments and in stable enhancer trap lines, suppression is achieved without compromising expression within the nervous system. Furthermore, in REx2 enhancer trap lines non-neuronal expression can be de-repressed by knocking down expression of the NRSE binding protein RE1-silencing transcription factor (Rest). In one line, we show that the resulting pattern of reporter gene expression coincides with that of the adjacent endogenous gene, hapln3. We demonstrate that three common basal promoters are susceptible to the effects of the REx2 element, suggesting that this method may be useful for confining expression from many other promoters to the nervous system. This technique enables neural specific targeting of reporter genes and thus will facilitate the use of transgenic methods to manipulate circuit function in freely behaving larvae.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.