ArticleJournal of bacteriology2013
Genome-wide screening with hydroxyurea reveals a link between nonessential ribosomal proteins and reactive oxygen species production.
Article in Journal of bacteriology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 22 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 1 synthesis or guideline pooled it, 36 citations in OpenAlex.
- Synthetic lethal mutants inJournal of bacteriology · 2023Pooled it
- Identification and profiling of novel metagenome assembled uncultivated virus genomes from human gut.Virology journal · 2025Article
- Revised mechanism of hydroxyurea-induced cell cycle arrest and an improved alternative.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Can antimicrobial blue light contribute to resistance development? Genome-wide analysis revealed aBL-protective genes inMicrobiology spectrum · 2024Article
- The absence of the queuosine tRNA modification leads to pleiotropic phenotypes revealing perturbations of metal and oxidative stress homeostasis in Escherichia coli K12.Metallomics : integrated biometal science · 2022Article
- Genome-wide CRISPR screens reveal cyclin C as synthetic survival target of BRCA2.Nucleic acids research · 2021Article
- Hydroxyurea-The Good, the Bad and the Ugly.Genes · 2021Review
- Protein structure, amino acid composition and sequence determine proteome vulnerability to oxidation-induced damage.The EMBO journal · 2020Article
- Rates of Molecular Evolution in a MarineViruses · 2019Article
- Regulation of acetate metabolism and coordination with the TCA cycle via a processed small RNA.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Quercetin enhances stress resistance inJournal of food science and technology · 2018Article
- Replication Rapidly Recovers and Continues in the Presence of Hydroxyurea in Escherichia coli.Journal of bacteriology · 2018Article
- Maladaptive DNA repair is the ultimate contributor to the death of trimethoprim-treated cells under aerobic and anaerobic conditions.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Heme deficiency sensitizes yeast cells to oxidative stress induced by hydroxyurea.The Journal of biological chemistry · 2017Article
- Article
- The Cell Killing Mechanisms of Hydroxyurea.Genes · 2016Review
- DNA replication inhibitor hydroxyurea alters Fe-S centers by producing reactive oxygen species in vivo.Scientific reports · 2016Article
- A Chemical-Genomic Screen of Neglected Antibiotics Reveals Illicit Transport of Kasugamycin and Blasticidin S.PLoS genetics · 2016Article
- Enzyme recruitment and its role in metabolic expansion.Biochemistry · 2014Review
- Central role for RNase YbeY in Hfq-dependent and Hfq-independent small-RNA regulation in bacteria.BMC genomics · 2014Article
Corrections and comments
- Erratum issued
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
We performed a screening of hydroxyurea (HU)-sensitive mutants using a single-gene-deletion mutant collection in Escherichia coli. HU inhibits ribonucleotide reductase (RNR), which leads to arrest of the replication fork. Surprisingly, the wild-type was less resistant to HU than the average for the Keio Collection. Respiration-defective mutants were significantly more resistant to HU, suggesting that the generation of reactive oxygen species (ROS) contributes to cell death. High-throughput screening revealed that 15 mutants were completely sensitive on plates containing 7.5 mM HU. Unexpectedly, translation-related mutants based on COG categorization were the most enriched, and three of them were deletion mutants of nonessential ribosomal proteins (L1, L32, and L36). We found that, in these mutants, an increased membrane stress response was provoked, resulting in increased ROS generation. The addition of OH radical scavenger thiourea rescued the HU sensitivity of these mutants, suggesting that ROS generation is the direct cause of cell death. Conversely, both the deletion of rpsF and the deletion of rimK, which encode S6 and S6 modification enzymes, respectively, showed an HU-resistant phenotype. These mutants increased the copy number of the p15A-based plasmid and exhibited reduced basal levels of SOS response. The data suggest that nonessential proteins indirectly affect the DNA-damaging process.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.