Evidence map›Paper›PMID 23292705›Full record

ReviewCurrent protocols in cytometry2013

Spectral flow cytometry.

John P Nolan, Danilo Condello

Open access · greenAbstract readHistorical ArticleReview
In one paragraph

Review in Current protocols in cytometry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 79 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
79citing papers in PubMed, 1 pooled it
4.6field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

79 citing papers in PubMed, 1 synthesis or guideline pooled it, 142 citations in OpenAlex.

  1. Pooled it
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  15. Spectral Flow Cytometry: The Current State and Future of the Technology.International journal of molecular sciences · 2025
    Review
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19 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

John P NolanLa Jolla Bioengineering Institute, San Diego, California.
Danilo CondelloLa Jolla Bioengineering Institute, San Diego, California.
La Jolla Bioengineering Institute · US

Funding

Raman Flow Cytometry for Diagnostics and Drug DiscoveryR01EB003824 · NIBIB · SCINTILLON INSTITUTE FOR PHOTOBIOLOGY · PI NOLAN, JOHN P · 2004 to 2014
$12.2M
NIBIB NIH HHS EB003824NIBIB NIH HHS R01 EB003824
6 · The paper itself

Abstract

Interest in measuring the complete fluorescence spectra of individual cells in flow can be traced to the earliest days of flow cytometry. Recent advances in detectors, optics, and computation have made it possible to make full spectral measurements in the sub-millisecond time frame in which flow cytometry measurements typically occur. This opens up new possibilities for applying spectroscopy to the analysis of individual cells. This unit reviews historical and contemporary approaches to spectral flow cytometry, as well as instrument design, calibration, and data analysis for spectral flow cytometry applications.

Indexed as

AnimalsCalibrationFlow CytometryHistory, 20th CenturyHistory, 21st CenturyHumansModels, BiologicalSingle-Cell AnalysisSpectrum Analysis, RamanStatistics as Topic

Identifiers

PMID23292705
PMCPMC3556726
OpenAlexW2159917050

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.