ArticleExperimental physiology2013
The mechanical uncoupler blebbistatin is associated with significant electrophysiological effects in the isolated rabbit heart.
Article in Experimental physiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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Who cites it
52 citing papers in PubMed, 82 citations in OpenAlex.
- A Beginner's Guide to Cardiac Optical Mapping.Cardiovascular engineering and technology · 2026Review
- Blebbistatin reduces calcium buffering in cardiomyocytes: Consequences for cellular electrophysiology.The Journal of physiology · 2026Article
- AI-enabled motion artefact correction to replace emission- and excitation-ratiometry in cardiac optical mapping: a proof-of-concept study.Cardiovascular research · 2026Article
- Combined emission ratiometry and motion tracking for optical mapping of contracting hearts: Validation with monophasic action potentials.The Journal of physiology · 2026Article
- A song of heads and tails: myosin II conformational regulation and filament dynamics shape force generation in non-muscle cells.Biophysical reviews · 2026Review
- Differential response to acute ischemia between isolated contracting hearts and hearts perfused with excitation-contraction uncouplers.American journal of physiology. Heart and circulatory physiology · 2026Article
- Disruption of ventricular activation by subthreshold delayed afterdepolarizations in RyR2-R420Q catecholaminergic polymorphic ventricular tachycardia.Journal of molecular and cellular cardiology plus · 2025Article
- NaInternational journal of cardiology. Heart & vasculature · 2025Article
- Ventricular arrhythmogenic remodelling in diet-induced metabolic syndrome driven by right-to-left regional differences in action potential duration and dominant frequency gradients.The Journal of physiology · 2025Article
- Sequence-Dependent Repolarization Is Modulated by Endogenous Action Potential Duration Gradients Rather Than Electrical Coupling in Ventricular Myocardium.Journal of the American Heart Association · 2025Article
- Quantification of Sarcoplasmic Reticulum CaCurrent protocols · 2024Article
- Male and female atria exhibit distinct acute electrophysiological responses to sex steroids.Journal of molecular and cellular cardiology plus · 2024Article
- Mannitol and hyponatremia regulate cardiac ventricular conduction in the context of sodium channel loss of function.American journal of physiology. Heart and circulatory physiology · 2024Article
- Vericiguat suppresses ventricular tachyarrhythmias inducibility in a rabbit myocardial infarction model.PloS one · 2024Article
- Virtual blebbistatin: A robust and rapid software approach to motion artifact removal in optical mapping of cardiomyocytes.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Autonomic neuro-cardiac profile of electrical, structural and neuronal remodeling in myocardial infarction-induced heart failure.Journal of molecular and cellular cardiology plus · 2023Article
- Optical mapping of contracting hearts.The Journal of physiology · 2023Review
- Assessment of Tissue Viability by Functional Imaging of Membrane Potential.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Optogenetic manipulation of cardiac repolarization gradients using sub-threshold illumination.Frontiers in physiology · 2023Article
- Heterogeneities in Ventricular Conduction Following Treatment with Heptanol: A Multi-Electrode Array Study in Langendorff-Perfused Mouse Hearts.Life (Basel, Switzerland) · 2022Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Blebbistatin (BS) is a recently discovered inhibitor of the myosin II isoform and has been adopted as the mechanical uncoupler of choice for optical mapping, because previous studies suggest that BS has no significant cardiac electrophysiological effects in a number of species. The aim of this study was to determine whether BS affects cardiac electrophysiology in isolated New Zealand White rabbit hearts. Langendorff-perfused hearts (n=39) in constant-flow mode had left ventricular monophasic action potential duration (MAPD) measured at apical and basal regions during constant pacing (300 ms cycle length). Standard action potential duration restitution was obtained using the single extrastimulus method with measurement of the maximal restitution slope. Ventricular fibrillation threshold was measured as the minimal current inducing sustained ventricular fibrillation with burst pacing (30 stimuli, at 30 ms intervals). Optical action potentials were recorded using the voltage-sensitive dye di-4-ANEPPS. Measurements were taken at baseline and after 60 min perfusion with BS (5 μm). Blebbistatin significantly prolonged left ventricular apical (mean±SEM; from 129.9±2.9 to 170.7±4.1 ms, P<0.001, n=8) and basal MAPD (from 135.0±2.3 to 163.3±5.6 ms, P<0.001) and effective refractory period (from 141.3±4.8 to 175.6±3.7 ms, P<0.001) whilst increasing the maximal slope of restitution (apex, from 0.79±0.09 to 1.57±0.16, P<0.001; and base, from 0.71±0.06 to 1.44±0.24, P<0.001) and ventricular fibrillation threshold (from 5.3±1.1 to 17.0±2.9 mA, P<0.001). In other hearts, blebbistatin significantly prolonged optically recorded action potentials (from 136.5±6.3 to 173.0±7.9 ms, P<0.05, n=4). In control experiments, the increase of MAPD with blebbistatin was present whether the hearts were perfused in constant-pressure mode (n=5) or in unloaded conditions (n=5). These data show that blebbistatin significantly affects cardiac electrophysiology. Its use in optical mapping studies should be treated with caution.
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