ArticleEuropean journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology2013
The gut microbiota of a patient with resistant tuberculosis is more comprehensively studied by culturomics than by metagenomics.
Article in European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 76 papers.
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76 citing papers in PubMed.
- High-throughput single-cell isolation ofMicrobiology spectrum · 2026Article
- Estimation of gut microbiome motif associated with active tuberculosis - A case control study.Bioinformation · 2026Article
- Determination of Gut Microbiome Patterns Associated with Developing Active Tuberculosis in a Case-Control Study.Journal of pharmacy & bioallied sciences · 2025Article
- Status and outlook of pulmonary tuberculosis coinfection.Journal of research in medical sciences : the official journal of Isfahan University of Medical Sciences · 2025Review
- Ethanol-ProducingMicroorganisms · 2023Article
- Therapeutically targeting the consequences of HIV-1-associated gastrointestinal dysbiosis: Implications for neurocognitive and affective alterations.Pharmacology, biochemistry, and behavior · 2023Article
- Maliibacterium massiliense gen. nov. sp. nov., Isolated from Human Feces and Proposal of Maliibacteriaceae fam. nov.Current microbiology · 2023Article
- Identification of lipid droplets in gut bacteria.Protein & cell · 2023Article
- Marine Bioprospecting, Biocatalysis and Process Development.Microorganisms · 2022Review
- Weizmannia faecalis sp. nov., isolated from a human stool sample.Archives of microbiology · 2022Article
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- Cultivating marine bacteria under laboratory conditions: Overcoming the "unculturable" dogma.Frontiers in bioengineering and biotechnology · 2022Article
- Clostridium culturomicium sp. nov. and Clostridium jeddahitimonense sp. nov., novel members of the Clostridium genus isolated from the stool of an obese Saudi Arabian.Current microbiology · 2021Article
- Gut Microbiota: the Emerging Link to Lung Homeostasis and Disease.Journal of bacteriology · 2021Review
- Shen-ling-bai-zhu-san ameliorates inflammation and lung injury by increasing the gut microbiota in the murine model of Streptococcus pneumonia-induced pneumonia.BMC complementary medicine and therapies · 2020Article
- Consolidation of Clinical Microbiology Laboratories and Introduction of Transformative Technologies.Clinical microbiology reviews · 2020Review
- Multi-Omics Approaches: The Key to Improving Respiratory Health in People With Cystic Fibrosis?Frontiers in pharmacology · 2020Review
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- Single-Anastomosis Duodenal Jejunal Bypass Improve Glucose Metabolism by Regulating Gut Microbiota and Short-Chain Fatty Acids in Goto-Kakisaki Rats.Frontiers in microbiology · 2020Article
16 more citing papers are in PubMed but not listed here.
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7 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Gut microbiota consists of 10(10) bacteria per gram of stool. Many antibiotic regimens induce a reduction in both the diversity and the abundance of the gut flora. We analyzed one stool sample collected from a patient treated for drug-resistant Mycobacterium tuberculosis and who ultimately died from pneumonia due to a Streptococcus pneumoniae 10 months later. We performed microscopic observation, used 70 culture conditions (microbial culturomics) with identification by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF) and 16S rRNA amplification and sequencing, pyrosequencing, and 18S rRNA amplification and clone sequencing. Electron and optical microscopic observations revealed the presence of yeast, but no bacterial species were observed. By culture, only 39 bacterial species were identified, including one new species, as well as three species that have not been previously observed in the human gut. The pyrosequencing showed only 18 phylotypes, detecting a lower number of bacterial species than the culture techniques. Only two phylotypes overlapped with culturomics. In contrast, an amount of chloroplasts was found. Additionally, specific molecular eukaryote detection found three fungal species. We recovered, for the first time, more cultivable than non-cultivable bacterial species in a patient with a low bacterial load in the gut, demonstrating the depth bias of pyrosequencing. We propose that the desertification of gut microbiota in this patient is a reflection of the total body microbiota and may have contributed to the invasive infection of S. pneumoniae. This finding suggests that caution should be applied when treating patients with broad-spectrum antibiotics, and preventive measures should be taken in order to avoid invasive infection.
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