ArticleEukaryotic cell2013
Bradyzoite pseudokinase 1 is crucial for efficient oral infectivity of the Toxoplasma gondii tissue cyst.
Article in Eukaryotic cell, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers, 2 of them syntheses that pooled it.
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Who cites it
32 citing papers in PubMed, 2 syntheses or guidelines pooled it, 52 citations in OpenAlex.
- Systematic review and meta-analysis of variation in Toxoplasma gondii cyst burden in the murine model.Experimental parasitology · 2019Pooled it
- Activities of anti-Toxoplasma drugs and compounds against tissue cysts in the last three decades (1987 to 2017), a systematic review.Parasitology research · 2018Pooled it
- Dense granule proteins: key mediators ofFrontiers in medicine · 2026Review
- Loss of NcBPK1 impairs bradyzoite differentiation and enhances virulence in Neospora caninum.Parasites & vectors · 2025Article
- Ribosome-Associated Quality Control Mediated by Rqc2 Contributes to the Lytic Cycle and Stage Conversion ofMicroorganisms · 2025Article
- A Novel Nuclear Protein Complex Controlling the Expression of Developmentally Regulated Genes in Toxoplasma Gondii.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Effective factors in the pathogenesis ofHeliyon · 2024Review
- The beta subunit of AMP-activated protein kinase is critical for cell cycle progression and parasite development in Toxoplasma gondii.Cellular and molecular life sciences : CMLS · 2022Article
- Phosphoglucomutase 1 contributes to optimal cyst development in Toxoplasma gondii.BMC research notes · 2022Article
- Released Parasite-Derived Kinases as Novel Targets for Antiparasitic Therapies.Frontiers in cellular and infection microbiology · 2022Review
- Toxoplasma gondii PPM3C, a secreted protein phosphatase, affects parasitophorous vacuole effector export.PLoS pathogens · 2020Article
- Pathophysiology of ocular toxoplasmosis: Facts and open questions.PLoS neglected tropical diseases · 2020Review
- The molecular biology and immune control of chronic Toxoplasma gondii infection.The Journal of clinical investigation · 2020Review
- Toxoplasma gondii metacaspase 2 is an important factor that influences bradyzoite formation in the Pru strain.Parasitology research · 2020Article
- Article
- The Toxoplasma gondii Cyst Wall Interactome.mBio · 2020Article
- Toxoplasma gondii: Bradyzoite Differentiation In Vitro and In Vivo.Methods in molecular biology (Clifton, N.J.) · 2020Article
- Proximity biotinylation reveals novel secreted dense granule proteins of Toxoplasma gondii bradyzoites.PloS one · 2020Article
- Article
- Divergent kinase regulates membrane ultrastructure of theProceedings of the National Academy of Sciences of the United States of America · 2019Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The tissue cyst formed by the bradyzoite stage of Toxoplasma gondii is essential for persistent infection of the host and oral transmission. Bradyzoite pseudokinase 1 (BPK1) is a component of the cyst wall, but nothing has previously been known about its function. Here, we show that immunoprecipitation of BPK1 from in vitro bradyzoite cultures, 4 days postinfection, identifies at least four associating proteins: MAG1, MCP4, GRA8, and GRA9. To determine the role of BPK1, a strain of Toxoplasma was generated with the bpk1 locus deleted. This BPK1 knockout strain (Δbpk1) was investigated in vitro and in vivo. No defect was found in terms of in vitro cyst formation and no difference in pathogenesis or cyst burden 4 weeks postinfection (wpi) was detected after intraperitoneal (i.p.) infection with Δbpk1 tachyzoites, although the Δbpk1 cysts were significantly smaller than parental or BPK1-complemented strains at 8 wpi. Pepsin-acid treatment of 4 wpi in vivo cysts revealed that Δbpk1 parasites are significantly more sensitive to this treatment than the parental and complemented strains. Consistent with this, 4 wpi Δbpk1 cysts showed reduced ability to cause oral infection compared to the parental and complemented strains. Together, these data reveal that BPK1 plays a crucial role in the in vivo development and infectivity of Toxoplasma cysts.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.