ArticleActa biomaterialia2013
Primary human chondrocyte extracellular matrix formation and phenotype maintenance using RGD-derivatized PEGDM hydrogels possessing a continuous Young's modulus gradient.
Article in Acta biomaterialia, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers, 1 of them a synthesis that pooled it.
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Who cites it
32 citing papers in PubMed, 1 synthesis or guideline pooled it, 72 citations in OpenAlex.
- Alteration in cartilage matrix stiffness as an indicator and modulator of osteoarthritis.Bioscience reports · 2024Pooled it
- Advancing urethral health research: Characterization of a male porcine urethra for lower urinary tract investigations.Histology and histopathology · 2026Article
- Three-Dimensional Bioprinting of Regenerative Cartilage Constructs with Directional Ionically Derived Stiffness Gradients.Journal of functional biomaterials · 2025Article
- Tough PEGDA-Based Bioadhesive Hydrogels for High-Strength In Situ Cartilage Adhesion.ACS omega · 2025Article
- Intermittent Catheters with Integrated Amphiphilic Surfactant Reduce Urethral Microtrauma in anJournal of functional biomaterials · 2025Article
- Advancements in hydrogel design for articular cartilage regeneration: A comprehensive review.Bioactive materials · 2025Review
- The Effect of Crosslinking Density on Nasal Chondrocytes' Redifferentiation.Annals of biomedical engineering · 2024Article
- Collagen fibril-like injectable hydrogels from self-assembled nanoparticles for promoting wound healing.Bioactive materials · 2024Article
- Polymeric Hydrogels for Controlled Drug Delivery to Treat Arthritis.Pharmaceutics · 2022Review
- Gradient Hydrogels for Optimizing Niche Cues to Enhance Cell-Based Cartilage Regeneration.Tissue engineering. Part A · 2021Article
- Advanced hydrogels for the repair of cartilage defects and regeneration.Bioactive materials · 2021Review
- Hydrogel Models with Stiffness Gradients for Interrogating Pancreatic Cancer Cell Fate.Bioengineering (Basel, Switzerland) · 2021Review
- Function and Mechanism of RGD in Bone and Cartilage Tissue Engineering.Frontiers in bioengineering and biotechnology · 2021Review
- Effect of Laminin Derived Peptides IKVAV and LRE Tethered to Hyaluronic Acid on hiPSC Derived Neural Stem Cell Morphology, Attachment and Neurite Extension.Journal of functional biomaterials · 2020Article
- In Vitro Evaluation of the Influence of Substrate Mechanics on Matrix-Assisted Human Chondrocyte Transplantation.Journal of functional biomaterials · 2020Article
- Impact of Hydrogel Stiffness on Differentiation of Human Adipose-Derived Stem Cell Microspheroids.Tissue engineering. Part A · 2019Article
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- Modulating Viscoelasticity, Stiffness, and Degradation of Synthetic Cellular Niches via Stoichiometric Tuning of Covalent versus Dynamic Noncovalent Cross-Linking.ACS central science · 2018Article
- Gradient Material Strategies for Hydrogel Optimization in Tissue Engineering Applications.High-throughput · 2018Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Efficient ex vivo methods for expanding primary human chondrocytes while maintaining the phenotype is critical to advancing the sourcing of autologous cells for tissue engineering applications. While there has been significant research reported in the literature, systematic approaches are necessary to determine and optimize the chemical and mechanical scaffold properties for hyaline cartilage generation using limited cell numbers. Functionalized hydrogels possessing continuous variations in physico-chemical properties are, therefore, an efficient three-dimensional platform for studying several properties simultaneously. Herein we describe a polyethylene glycol dimethacrylate (PEGDM) hydrogel system with a modulus gradient (~27,000-3800 Pa) containing a uniform concentration of arginine-glycine-aspartic acid (RGD) peptide to enhance cell adhesion in order to correlate primary human osteoarthritic chondrocyte proliferation, phenotype maintenance, and extracellular matrix (ECM) production with hydrogel properties. Cell number and chondrogenic phenotype (CD14:CD90 ratios) were found to decline in regions with a higher storage modulus (>13,100 Pa), while regions with a lower storage modulus maintained their cell number and phenotype. Over 3 weeks culture hydrogel regions possessing a lower Young's modulus experienced an increase in ECM content (~200%) compared with regions with a higher storage modulus. Variations in the amount and organization of the cytoskeletal markers actin and vinculin were observed within the modulus gradient, which are indicative of differences in chondrogenic phenotype maintenance and ECM expression. Thus scaffold mechanical properties have a significant impact in modulating human osteoarthritic chondrocyte behavior and tissue formation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.