ArticleJournal of the American Society of Nephrology : JASN2013
An in vivo functional analysis system for renal gene discovery in Drosophila pericardial nephrocytes.
Article in Journal of the American Society of Nephrology : JASN, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
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62 citing papers in PubMed, 110 citations in OpenAlex.
- Article
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- HIV-1 Nef synergizes with APOL1-G1 to induce nephrocyte cell death in HIV-related kidney diseases.Disease models & mechanisms · 2025Article
- A Drug Screening Platform for Podocytopathies in Drosophila Nephrocytes.Journal of the American Society of Nephrology : JASN · 2025Article
- Piezo, Nephrocyte Function, and Slit Diaphragm Maintenance in Drosophila.Journal of the American Society of Nephrology : JASN · 2025Article
- The nutrient sensor CRTC and Sarcalumenin/thinman represent an alternate pathway in cardiac hypertrophy.Cell reports · 2024Article
- Promoting mitochondrial dynamics by inhibiting the PINK1-PRKN pathway to relieve diabetic nephropathy.Disease models & mechanisms · 2024Article
- AbioRxiv : the preprint server for biology · 2024Article
- APOL1-G2 accelerates nephrocyte cell death by inhibiting the autophagy pathway.Disease models & mechanisms · 2023Article
- A SNARE protective pool antagonizes APOL1 renal toxicity in Drosophila nephrocytes.Cell & bioscience · 2023Article
- Single-cell profiling of the developing embryonic heart in Drosophila.Development (Cambridge, England) · 2023Article
- Mapping genomic regulation of kidney disease and traits through high-resolution and interpretable eQTLs.Nature communications · 2023Article
- Modeling ofKidney international reports · 2023Article
- JiangShi(): a widely distributed Mucin-like protein essential for Drosophila development.G3 (Bethesda, Md.) · 2022Article
- Drosophila melanogaster: a simple genetic model of kidney structure, function and disease.Nature reviews. Nephrology · 2022Review
- Transcriptional and functional motifs defining renal function revealed by single-nucleus RNA sequencing.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- PI(4,5)P2 controls slit diaphragm formation and endocytosis in Drosophila nephrocytes.Cellular and molecular life sciences : CMLS · 2022Article
- Physiological ROS controls Upd3-dependent modeling of ECM to support cardiac function inScience advances · 2022Article
- Rap1 Activity Is Essential for Focal Adhesion and Slit Diaphragm Integrity.Frontiers in cell and developmental biology · 2022Article
- UsingFrontiers in cell and developmental biology · 2022Review
2 more citing papers are in PubMed but not listed here.
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3 authors at 1 institution in 1 country.
Funding
Abstract
The difficulty in accessing mammalian nephrons in vivo hinders the study of podocyte biology. The Drosophila nephrocyte shares remarkable similarities to the glomerular podocyte, but the lack of a functional readout for nephrocytes makes it challenging to study this model of the podocyte, which could potentially harness the power of Drosophila genetics. Here, we present a functional analysis of nephrocytes and establish an in vivo system to screen for renal genes. We found that nephrocytes efficiently take up secreted fluorescent protein, and therefore, we generated a transgenic line carrying secreted fluorescent protein and combined it with a nephrocyte-specific driver for targeted gene knockdown, allowing the identification of genes required for nephrocyte function. To validate this system, we examined the effects of knocking down sns and duf, the Drosophila homologs of nephrin and Neph1, respectively, in pericardial nephrocytes. Knockdown of sns or duf completely abolished the accumulation of the fluorescent protein in pericardial nephrocytes. Examining the ultrastructure revealed that the formation of the nephrocyte diaphragm and lacunar structure, which is essential for protein uptake, requires sns. Our preliminary genetic screen also identified Mec2, which encodes the homolog of mammalian Podocin. Taken together, these data suggest that the Drosophila pericardial nephrocyte is a useful in vivo model to help identify genes involved in podocyte biology and facilitate the discovery of renal disease genes.
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