ArticleCurrent biology : CB2013
Mechanism and regulation of Cdc25/Twine protein destruction in embryonic cell-cycle remodeling.
Article in Current biology : CB, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed.
- The maternal-to-zygotic transition: reprogramming of the cytoplasm and nucleus.Nature reviews. Genetics · 2025Review
- Cell state transitions are decoupled from cell division during early embryo development.Nature cell biology · 2024Article
- Collective effects of cell cleavage dynamics.Frontiers in cell and developmental biology · 2024Review
- Review
- Structure-function analysis of Cdc25Fly · 2022Article
- Temporal control of late replication and coordination of origin firing by self-stabilizing Rif1-PP1 hubs inProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Modeling the role for nuclear import dynamics in the early embryonic cell cycle.Biophysical journal · 2021Article
- Cell cycle control during early embryogenesis.Development (Cambridge, England) · 2021Article
- The Atr-Chek1 pathway inhibits axon regeneration in response to Piezo-dependent mechanosensation.Nature communications · 2021Article
- Temporal Gradients Controlling Embryonic Cell Cycle.Biology · 2021Review
- The nuclear to cytoplasmic ratio directly regulates zygotic transcription inProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Control of Cell Growth and Proliferation by the Tribbles Pseudokinase: Lessons from Drosophila.Cancers · 2021Review
- Interphase-arrested Drosophila embryos activate zygotic gene expression and initiate mid-blastula transition events at a low nuclear-cytoplasmic ratio.PLoS biology · 2020Article
- Fluorescence fluctuation analysis reveals PpV dependent Cdc25 protein dynamics in living embryos.PLoS genetics · 2020Article
- Multiple Functions of the Essential GeneG3 (Bethesda, Md.) · 2019Article
- The role of dNTP metabolites in control of the embryonic cell cycle.Cell cycle (Georgetown, Tex.) · 2019Review
- Histone concentration regulates the cell cycle and transcription in early development.Development (Cambridge, England) · 2019Article
- Metabolic Regulation of Developmental Cell Cycles and Zygotic Transcription.Current biology : CB · 2019Article
- Mechanisms regulating zygotic genome activation.Nature reviews. Genetics · 2019Review
- Regulatory principles governing the maternal-to-zygotic transition: insights from Drosophila melanogaster.Open biology · 2018Review
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Abstract
backgroundIn Drosophila embryos, the midblastula transition (MBT) dramatically remodels the cell cycle during the 14(th) interphase. Before the MBT, each cycle is composed of only a short S phase and mitosis. At the MBT, S phase is dramatically lengthened by the onset of late replication, and a G2 phase is introduced. Both changes set the stage for gastrulation and require downregulation of Cdc25 phosphatase, which was previously attributed to the elimination of its transcripts at the MBT.
resultsPremature removal of cdc25 transcripts by RNAi did not affect progression to the MBT. Instead, an antibody against the Cdc25 isoform Twine showed that Twine protein was abundant and stable until the MBT, when it was destabilized and rapidly eliminated. Persistence of pre-MBT levels of Twine was sufficient to prevent cell-cycle slowing. Twine protein destruction was timed by the nucleocytoplasmic ratio and depended on the activation of zygotic transcription at the MBT, including expression of the gene tribbles, whose activity was sufficient to trigger Twine destruction and was required for prompt Twine disappearance.
conclusionsWe propose that the developmentally regulated destruction of Twine protein is a critical switch that contributes to the cell-cycle change at the MBT, including the addition of a G2 phase and onset of late replication. Moreover, we show that this destruction is triggered by the nucleocytoplasmic ratio-dependent onset of zygotic transcription of tribbles and other unknown genes.
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