ArticleCell communication and signaling : CCS2013
Structural and functional characteristics of cGMP-dependent methionine oxidation in Arabidopsis thaliana proteins.
Article in Cell communication and signaling : CCS, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 122 citations in OpenAlex.
- Reactive Oxygen Species in Plants: Metabolism, Signaling, and Oxidative Modifications.Antioxidants (Basel, Switzerland) · 2025Review
- Accurate Proteomewide Measurement of Methionine Oxidation in Aging Mouse Brains.Journal of proteome research · 2022Article
- (De)Activation (Ir)Reversibly or Degradation: Dynamics of Post-Translational Protein Modifications in Plants.Life (Basel, Switzerland) · 2022Review
- Citrullination of Proteins as a Specific Response Mechanism in Plants.Frontiers in plant science · 2021Article
- Susceptibility of Protein Methionine Oxidation in Response to Hydrogen Peroxide Treatment-Ex Vivo Versus In Vitro: A Computational Insight.Antioxidants (Basel, Switzerland) · 2020Article
- Rapid Quantification of Peptide Oxidation Isomers From Complex Mixtures.Analytical chemistry · 2020Article
- Changes in the Arabidopsis RNA-binding proteome reveal novel stress response mechanisms.BMC plant biology · 2019Article
- Drought Stress Causes Specific Changes to the Spliceosome and Stress Granule Components.Frontiers in molecular biosciences · 2019Article
- Physiological Roles of Plant Methionine Sulfoxide Reductases in Redox Homeostasis and Signaling.Antioxidants (Basel, Switzerland) · 2018Review
- Comparative Membrane-Associated Proteomics of Three Different Immune Reactions in Potato.International journal of molecular sciences · 2018Article
- Downstream Targets of Cyclic Nucleotides in Plants.Frontiers in plant science · 2018Article
- Discovery of Novel Functional Centers With Rationally Designed Amino Acid Motifs.Computational and structural biotechnology journal · 2018Review
- A machine learning approach for predicting methionine oxidation sites.BMC bioinformatics · 2017Article
- Article
- Cyclic Nucleotide Monophosphates and Their Cyclases in Plant Signaling.Frontiers in plant science · 2017Review
- Relative Quantification of Sites of Peptide and Protein Modification Using Size Exclusion Chromatography Coupled with Electron Transfer Dissociation.Journal of the American Society for Mass Spectrometry · 2016Article
- Sulphur Atoms from Methionines Interacting with Aromatic Residues Are Less Prone to Oxidation.Scientific reports · 2015Article
- A peptide N-terminal protection strategy for comprehensive glycoproteome analysis using hydrazide chemistry based method.Scientific reports · 2015Article
- Convergent signaling pathways--interaction between methionine oxidation and serine/threonine/tyrosine O-phosphorylation.Cell stress & chaperones · 2015Article
- Proteomic analysis of crop plants under abiotic stress conditions: where to focus our research?Frontiers in plant science · 2015Article
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
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backgroundIncreasing structural and biochemical evidence suggests that post-translational methionine oxidation of proteins is not just a result of cellular damage but may provide the cell with information on the cellular oxidative status. In addition, oxidation of methionine residues in key regulatory proteins, such as calmodulin, does influence cellular homeostasis. Previous findings also indicate that oxidation of methionine residues in signaling molecules may have a role in stress responses since these specific structural modifications can in turn change biological activities of proteins.
findingsHere we use tandem mass spectrometry-based proteomics to show that treatment of Arabidopsis thaliana cells with a non-oxidative signaling molecule, the cell-permeant second messenger analogue, 8-bromo-3,5-cyclic guanosine monophosphate (8-Br-cGMP), results in a time-dependent increase in the content of oxidised methionine residues. Interestingly, the group of proteins affected by cGMP-dependent methionine oxidation is functionally enriched for stress response proteins. Furthermore, we also noted distinct signatures in the frequency of amino acids flanking oxidised and un-oxidised methionine residues on both the C- and N-terminus.
conclusionsGiven both a structural and functional bias in methionine oxidation events in response to a signaling molecule, we propose that these are indicative of a specific role of such post-translational modifications in the direct or indirect regulation of cellular responses. The mechanisms that determine the specificity of the modifications remain to be elucidated.
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Registered trials
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