ArticleInternational journal of biological sciences2013
Identification of microRNAs in Helicoverpa armigera and Spodoptera litura based on deep sequencing and homology analysis.
Article in International journal of biological sciences, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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22 citing papers in PubMed, 52 citations in OpenAlex.
- A Comprehensive Larval microRNA Atlas ofInternational journal of molecular sciences · 2026Article
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- A Genetic Compensation Phenomenon and Global Gene Expression Changes in Sex-miR-2766-3p Knockout Strain ofInsects · 2022Article
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- miR-34 regulates larval growth and wing morphogenesis by directly modulating ecdysone signalling and cuticle protein inRNA biology · 2020Article
- Differential expression of novel MicroRNAs from developing fetal heart of Gallus gallus domesticus implies a role in cardiac development.Molecular and cellular biochemistry · 2019Article
- The Role of MicroRNAs in Genome Response to Plant-Lepidoptera Interaction.Plants (Basel, Switzerland) · 2019Review
- Genome-wide identification, expression profiling, and target gene analysis of microRNAs in the Onion thrips,Ecology and evolution · 2018Article
- Identification of conserved miRNA molecules in einkorn wheat (Triticum monococcum subsp. monococcum) by using small RNA sequencing analysis.Turkish journal of biology = Turk biyoloji dergisi · 2018Article
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- Identification and Evaluation of Suitable Reference Genes for Normalization of MicroRNA Expression in Helicoverpa armigera (Lepidoptera: Noctuidae) Using Quantitative Real-Time PCR.Journal of insect science (Online) · 2017Article
- Article
- Comparative profiling of microRNAs in the winged and wingless English grain aphid, Sitobion avenae (F.) (Homoptera: Aphididae).Scientific reports · 2016Article
- Article
- Identification and characteristics of microRNAs from army worm, Spodoptera frugiperda cell line Sf21.PloS one · 2015Article
- Identification of Novel and Conserved microRNAs in Homalodisca vitripennis, the Glassy-Winged Sharpshooter by Expression Profiling.PloS one · 2015Article
- Identification and characterization of microRNAs in pearl oyster Pinctada martensii by Solexa deep sequencing.Marine biotechnology (New York, N.Y.) · 2014Article
- Development associated profiling of chitinase and microRNA of Helicoverpa armigera identified chitinase repressive microRNA.Scientific reports · 2013Article
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The current identification of microRNAs (miRNAs) in insects is largely dependent on genome sequences. However, the lack of available genome sequences inhibits the identification of miRNAs in various insect species. In this study, we used a miRNA database of the silkworm Bombyx mori as a reference to identify miRNAs in Helicoverpa armigera and Spodoptera litura using deep sequencing and homology analysis. Because all three species belong to the Lepidoptera, the experiment produced reliable results. Our study identified 97 and 91 conserved miRNAs in H. armigera and S. litura, respectively. Using the genome of B. mori and BAC sequences of H. armigera as references, 1 novel miRNA and 8 novel miRNA candidates were identified in H. armigera, and 4 novel miRNA candidates were identified in S. litura. An evolutionary analysis revealed that most of the identified miRNAs were insect-specific, and more than 20 miRNAs were Lepidoptera-specific. The investigation of the expression patterns of miR-2a, miR-34, miR-2796-3p and miR-11 revealed their potential roles in insect development. miRNA target prediction revealed that conserved miRNA target sites exist in various genes in the 3 species. Conserved miRNA target sites for the Hsp90 gene among the 3 species were validated in the mammalian 293T cell line using a dual-luciferase reporter assay. Our study provides a new approach with which to identify miRNAs in insects lacking genome information and contributes to the functional analysis of insect miRNAs.
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