ArticleGenetics2013
Efficient targeted mutagenesis in medaka using custom-designed transcription activator-like effector nucleases.
Article in Genetics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed, 114 citations in OpenAlex.
- Catfish Transgenesis and Gene Editing: Overview, Recent Advances, and Future Perspectives.Biology · 2026Review
- A gene regulatory network combining Pax3/7, Sox10 and Mitf generates diverse pigment cell types in medaka and zebrafish.Development (Cambridge, England) · 2023Article
- Fish Models for Exploring Mitochondrial Dysfunction Affecting Neurodegenerative Disorders.International journal of molecular sciences · 2023Review
- Medaka, Oryzias latipes, egg envelopes are created by ovarian-expressed ZP proteins and liver-expressed choriogenins.Zoological letters · 2022Article
- Zebrafish, Medaka and Turquoise Killifish for Understanding Human Neurodegenerative/Neurodevelopmental Disorders.International journal of molecular sciences · 2022Review
- Targeted deletion of liver-expressed Choriogenin L results in the production of soft eggs and infertility in medaka, Oryzias latipes.Zoological letters · 2022Article
- Oxidative Stress Causes Masculinization of Genetically Female Medaka Without Elevating Cortisol.Frontiers in endocrinology · 2022Article
- Sexually dimorphic role of oxytocin in medaka mate choice.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Renal lesions in leptin receptor-deficient medaka (Journal of toxicologic pathology · 2019Article
- Targeted mutagenesis of the ryanodine receptor by Platinum TALENs causes slow swimming behaviour in Pacific bluefin tuna (Thunnus orientalis).Scientific reports · 2019Article
- Article
- Heat shock factor 1 protects germ cell proliferation during early ovarian differentiation in medaka.Scientific reports · 2019Article
- Identification and expression of the medaka inhibin βE subunit.Molecular biology reports · 2019Article
- A Collection of Transgenic Medaka Strains for Efficient Site-Directed Transgenesis Mediated by phiC31 Integrase.G3 (Bethesda, Md.) · 2018Article
- Distinct interactions of Sox5 and Sox10 in fate specification of pigment cells in medaka and zebrafish.PLoS genetics · 2018Article
- Production of the medaka derived from vitrified whole testes by germ cell transplantation.Scientific reports · 2017Article
- An efficient system for homology-dependent targeted gene integration in medaka (Zoological letters · 2017Article
- TALEN-mediated knock-in via non-homologous end joining in the crustacean Daphnia magna.Scientific reports · 2016Article
- Genome Editing in Astyanax mexicanus Using Transcription Activator-like Effector Nucleases (TALENs).Journal of visualized experiments : JoVE · 2016Article
- Mate-guarding behavior enhances male reproductive success via familiarization with mating partners in medaka fish.Frontiers in zoology · 2016Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transcription activator-like effector nucleases (TALENs) have become powerful tools for targeted genome editing. Here we demonstrate efficient targeted mutagenesis in medaka (Oryzias latipes), which serves as an excellent vertebrate model for genetics and genomics. We designed and constructed a pair of TALENs targeting the medaka DJ-1 gene, a homolog of human DJ-1 (PARK7). These TALENs induced a number of insertions and deletions in the injected embryos with extremely high efficiency. This induction of mutations occurred in a dose-dependent manner. All screened G0 fish injected with the TALENs transmitted the TALEN-induced mutations to the next generation with high efficiency (44-100%). We also confirmed that these TALENs induced site-specific mutations because none of the mutations were found at potential off-target sites. In addition, the DJ-1 protein was lost in DJ-1(Δ7/Δ7) fish that carried a TALEN-induced frameshift mutation in both alleles. We also investigated the effect of the N- and C-terminal regions of the transcription activator-like (TAL) effector domain on the gene-disrupting activity of DJ1-TALENs and found that 287 amino acids at the N terminus and 63 amino acids at the C terminus of the TAL domain exhibited the highest disrupting activity in the injected embryos. Our results suggest that TALENs enable us to rapidly and efficiently establish knockout medaka strains. This is the first report of targeted mutagenesis in medaka using TALENs. The TALEN technology will expand the potential of medaka as a model system for genetics and genomics.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.