ArticleNature protocols2013
Cytometric fingerprinting for analyzing microbial intracommunity structure variation and identifying subcommunity function.
Article in Nature protocols, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
What it found
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Who cites it
56 citing papers in PubMed.
- A Step-by-Step Guide to Successful Multiparametric Microbiota Flow Cytometry.Current protocols · 2026Article
- Establishing and analyzing the Simplified Human Intestinal Microbiota (SIHUMI) as a versatile in vitro gut microbiome model with qPCR-based strain-level tracking.Nature protocols · 2026Review
- Interindividual differences in acidogenic potential of the salivary microbiome after mouthwash exposure.BMC microbiology · 2026Article
- Single-cell microbiota phenotyping reveals distinct disease and therapy-associated signatures in Crohn's disease.Gut microbes · 2025Article
- Long-term TCDD oral exposure changes gut microbial glycosylation patterns in female but not male C57BL/6 mice.Archives of toxicology · 2025Article
- Integrating taxonomic and phenotypic information through FISH-enhanced flow cytometry for microbial community dynamics analysis.Microbiology spectrum · 2025Article
- Comparison of flow cytometry and heterotrophic plate count methods for dialysis water microbial monitoring.Scientific reports · 2025Article
- Resolving spatiotemporal dynamics in bacterial multicellular populations: approaches and challenges.Microbiology and molecular biology reviews : MMBR · 2025Review
- Quantifying synthetic bacterial community composition with flow cytometry: efficacy in mock communities and challenges in co-cultures.mSystems · 2025Article
- Unlocking the mechanism of action: a cost-effective flow cytometry approach for accelerating antimicrobial drug development.Microbiology spectrum · 2024Article
- Article
- Combining Flow Cytometry and Metagenomics Improves Recovery of Metagenome-Assembled Genomes in a Cell Culture from Activated Sludge.Microorganisms · 2023Article
- Opportunities in optical and electrical single-cell technologies to study microbial ecosystems.Frontiers in microbiology · 2023Review
- Exploration of microbiome diversity of stacked fermented grains by flow cytometry and cell sorting.Frontiers in microbiology · 2023Article
- Stabilizing microbial communities by looped mass transfer.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Advances in automated real-time flow cytometry for monitoring of bioreactor processes.Engineering in life sciences · 2022Review
- Validating Flow Cytometry as a Method for QuantifyingMicrobiology spectrum · 2022Article
- Cybergenetic control of microbial community composition.Frontiers in bioengineering and biotechnology · 2022Review
- Tracking defined microbial communities by multicolor flow cytometry reveals tradeoffs between productivity and diversity.Frontiers in microbiology · 2022Article
- Microbial disruption in the gut promotes cerebral endothelial dysfunction.Physiological reports · 2021Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Functions of complex natural microbial communities are realized by single cells that contribute differently to the overall performance of a community. Usually, molecular and, more recently, deep-sequencing techniques are used for detailed but resource-consuming phylogenetic or functional analyses of microbial communities. Here we present a method for analyzing dynamic community structures that rapidly detects functional (rather than phylogenetic) coherent subcommunities by monitoring changes in cell-specific and abiotic microenvironmental parameters. The protocol involves the use of flow cytometry to analyze elastic light scattering and fluorescent cell labeling, with subsequent determination of cell gate abundance and finally the creation of a cytometric community fingerprint. Abiotic parameter analysis data are correlated with the dynamic cytometric fingerprint to obtain a time-bound functional heat map. The map facilitates the identification of activity hot spots in communities, which can be further resolved by subsequent cell sorting of key subcommunities and concurrent phylogenetic analysis (terminal restriction fragment length polymorphism, tRFLP). The cytometric fingerprint information is based on gate template settings and the functional heat maps are created using an R script. Cytometric fingerprinting and evaluation can be accomplished in 1 d, and additional subcommunity composition information can be obtained in a further 6 d.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.