ArticleArteriosclerosis, thrombosis, and vascular biology2013
Analysis of stromal cell secretomes reveals a critical role for stromal cell-derived hepatocyte growth factor and fibronectin in angiogenesis.
Article in Arteriosclerosis, thrombosis, and vascular biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
30 citing papers in PubMed, 53 citations in OpenAlex.
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- Single-cell sorting based on secreted products for functionally defined cell therapies.Microsystems & nanoengineering · 2022Review
- Stem cell-based vascularization of microphysiological systems.Stem cell reports · 2021Review
- Transient Support from Fibroblasts is Sufficient to Drive Functional Vascularization in Engineered Tissues.Advanced functional materials · 2020Article
- Therapeutic application of adipose-derived stromal vascular fraction in diabetic foot.Stem cell research & therapy · 2020Review
- Microvessel Network Formation and Interactions with Pancreatic Islets in Three-Dimensional Chip Cultures.Tissue engineering. Part A · 2020Article
- Article
- Differential functional roles of fibroblasts and pericytes in the formation of tissue-engineered microvascular networks in vitro.NPJ Regenerative medicine · 2020Article
- Balance of interstitial flow magnitude and vascular endothelial growth factor concentration modulates three-dimensional microvascular network formation.APL bioengineering · 2019Article
- Investigating the potential of the secretome of mesenchymal stem cells derived from sickle cell disease patients.PloS one · 2019Article
- Engineering of vascularized 3D cell constructs to model cellular interactions through a vascular network.Biomicrofluidics · 2018Article
- Perfusable Vascular Network with a Tissue Model in a Microfluidic Device.Journal of visualized experiments : JoVE · 2018Article
- TGF-β1-induced differentiation of SHED into functional smooth muscle cells.Stem cell research & therapy · 2017Article
- Assessing angiogenic responses induced by primary human prostate stromal cells in a three-dimensional fibrin matrix assay.Oncotarget · 2016Article
- Combination scaffolds of salmon fibrin, hyaluronic acid, and laminin for human neural stem cell and vascular tissue engineering.Acta biomaterialia · 2016Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
Abstract
objectiveAngiogenesis requires tightly coordinated crosstalk between endothelial cells (ECs) and stromal cells, such as fibroblasts and smooth muscle cells. The specific molecular mechanisms moderating this process are still poorly understood. METHODS AND
resultsStromal cell-derived factors are essential for EC sprouting and lumen formation. We therefore compared the abilities of 2 primary fibroblast isolates and a primary smooth muscle cell isolate to promote in vitro angiogenesis, and analyzed their secretomes using a combination of nano liquid chromatography-mass spectrometry/mass spectrometry, quantitative PCR, and ELISA. Each isolate exhibited a different level of angiogenic ability. Using quantitative MS, we then compared the secretomes of a fibroblast isolate exhibiting low angiogenic activity, a fibroblast isolate exhibiting high angiogenic activity, and human umbilical vein ECs. High angiogenic fibroblast supernatants exhibited an overabundance of proteins associated with extracellular matrix constituents compared with low angiogenic fibroblasts or ECs. Finally, small interfering RNA technology and purified protein were used to confirm a role for stromal cell-derived hepatocyte growth factor and fibronectin in inducing EC sprouting.
conclusionsDifferences in stromal cell ability to induce angiogenesis are a result of differences in the secreted proteomes of both extracellular matrix proteins and proangiogenic growth factors.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.