ArticleJournal of radiation research2013
SPICE-NIRS microbeam: a focused vertical system for proton irradiation of a single cell for radiobiological research.
Article in Journal of radiation research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Review
- Primary and Secondary Bystander Effects of Proton Microbeam Irradiation on Human Lung Cancer Cells under Hypoxic Conditions.Biology · 2023Article
- Article
- Stimulation of Nuclear Factor (Erythroid-Derived 2)-like 2 Signaling by Nucleus Targeted Irradiation with Proton Microbeam.Biology · 2023Article
- Review
- A simple microscopy setup for visualizing cellular responses to DNA damage at particle accelerator facilities.Scientific reports · 2021Article
- Scaling parameter of the lethal effect of mammalian cells based on radiation-induced OH radicals: effectiveness of direct action in radiation therapy.Journal of radiation research · 2021Article
- Biologic Impact of Different Ultra-Low-Fluence Irradiations in Human Fibroblasts.Life (Basel, Switzerland) · 2020Article
- DNA damage response in prostate cancer cells by proton microbeam irradiation.Translational cancer research · 2020Article
- Nuclear factor (erythroid-derived 2)-like 2 antioxidative response mitigates cytoplasmic radiation-induced DNA double-strand breaks.Cancer science · 2019Article
- Single α-particle irradiation permits real-time visualization of RNF8 accumulation at DNA damaged sites.Scientific reports · 2017Article
- Chromothripsis-like chromosomal rearrangements induced by ionizing radiation using proton microbeam irradiation system.Oncotarget · 2016Article
- Co-visualization of DNA damage and ion traversals in live mammalian cells using a fluorescent nuclear track detector.Journal of radiation research · 2015Article
- Applications of High-Throughput Clonogenic Survival Assays in High-LET Particle Microbeams.Frontiers in oncology · 2015Article
Corrections and comments
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Authors and funding
14 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Single Particle Irradiation system to Cell (SPICE) facility at the National Institute of Radiological Sciences (NIRS) is a focused vertical microbeam system designed to irradiate the nuclei of adhesive mammalian cells with a defined number of 3.4 MeV protons. The approximately 2-μm diameter proton beam is focused with a magnetic quadrupole triplet lens and traverses the cells contained in dishes from bottom to top. All procedures for irradiation, such as cell image capturing, cell recognition and position calculation, are automated. The most distinctive characteristic of the system is its stability and high throughput; i.e. 3000 cells in a 5 mm × 5 mm area in a single dish can be routinely irradiated by the 2-μm beam within 15 min (the maximum irradiation speed is 400 cells/min). The number of protons can be set as low as one, at a precision measured by CR-39 detectors to be 99.0%. A variety of targeting modes such as fractional population targeting mode, multi-position targeting mode for nucleus irradiation and cytoplasm targeting mode are available. As an example of multi-position targeting irradiation of mammalian cells, five fluorescent spots in a cell nucleus were demonstrated using the γ-H2AX immune-staining technique. The SPICE performance modes described in this paper are in routine use. SPICE is a joint-use research facility of NIRS and its beam times are distributed for collaborative research.
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Registered trials
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