ArticleThe EMBO journal1990
Transcription factor Oct-2A contains functionally redundant activating domains and works selectively from a promoter but not from a remote enhancer position in non-lymphoid (HeLa) cells.
Article in The EMBO journal, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
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Who cites it
62 citing papers in PubMed, 145 citations in OpenAlex.
- Article
- Oct-2 forms a complex with Oct-1 on the iNOS promoter and represses transcription by interfering with recruitment of RNA PolII by Oct-1.Nucleic acids research · 2015Article
- Dissecting the role of distinct OCT4-SOX2 heterodimer configurations in pluripotency.Scientific reports · 2015Article
- Roles of two activation domains in Zap1 in the response to zinc deficiency in Saccharomyces cerevisiae.The Journal of biological chemistry · 2011Article
- Characterization of an Oct1 orthologue in the channel catfish, Ictalurus punctatus: a negative regulator of immunoglobulin gene transcription?BMC molecular biology · 2007Article
- Coactivator OBF-1 makes selective contacts with both the POU-specific domain and the POU homeodomain and acts as a molecular clamp on DNA.Molecular and cellular biology · 1998Article
- The different alternatively spliced isoforms of the Oct-2 transcription factor repress the involucrin promoter in a cell type-specific manner.Molecular biology reports · 1998Article
- Coactivation by OCA-B: definition of critical regions and synergism with general cofactors.Molecular and cellular biology · 1998Article
- Adjacent proline residues in the inhibitory domain of the Oct-2 transcription factor play distinct functional roles.Nucleic acids research · 1998Article
- POU domain transcription factors in embryonic development.Molecular biology reports · 1997Review
- The octamer-binding proteins Oct-1 and Oct-2 repress the HIV long terminal repeat promoter and its transactivation by Tat.The Biochemical journal · 1997Article
- The carboxy-terminal transactivation domain of Oct-4 acquires cell specificity through the POU domain.Molecular and cellular biology · 1997Article
- Transcriptional repression by RING finger protein TIF1 beta that interacts with the KRAB repressor domain of KOX1.Nucleic acids research · 1996Article
- CRISP-3, a protein with homology to plant defense proteins, is expressed in mouse B cells under the control of Oct2.Molecular and cellular biology · 1996Article
- The POU domain of SCIP/Tst-1/Oct-6 is sufficient for activation of an acetylcholine receptor promoter.Molecular and cellular biology · 1996Article
- The basic domain/leucine zipper protein hXBP-1 preferentially binds to and transactivates CRE-like sequences containing an ACGT core.Nucleic acids research · 1996Article
- Gene structure and characterization of the murine homologue of the B cell-specific transcriptional coactivator OBF-1.Nucleic acids research · 1996Article
- Cloning, functional characterization, and mechanism of action of the B-cell-specific transcriptional coactivator OCA-B.Molecular and cellular biology · 1995Article
- Functional domains of the heavy metal-responsive transcription regulator MTF-1.Nucleic acids research · 1995Article
- High mobility group protein 2 functionally interacts with the POU domains of octamer transcription factors.The EMBO journal · 1995Article
2 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In non-lymphoid cells such as HeLa cells, ectopic expression of the lymphocyte-specific transcription factor Oct-2A can activate reporter genes whose promoters consist of a single octamer sequence (ATTTGCAT) upstream of a TATA box. While the factor is strongly active in a promoter position, it tails as an enhancer factor: an enhancer consisting of multiple copies of the octamer sequence placed downstream of the reporter gene is not active in HeLa cells, even at high concentration of Oct-2A. In B lymphoid cells, however, the same enhancer is highly active. This could mean that an additional factor is required for enhancer activation in B cells. Furthermore, we have tested the transcriptional activation potential of Oct-2A with a series of N-terminal and C-terminal deletions. We show that a glutamine-rich domain near the N-terminus is required for full activity. Otherwise, large segments of the N-terminal half or the entire C-terminal region are dispensable in our assay, as long as the deletions do not impinge on the conserved POU domain which is sufficient for DNA binding. While N-terminal and C-terminal regions can functionally compensate for each other, a combined deletion that only retains the POU domain is a strong down mutation. We also find that activity depends on the promoter structure of the reporter gene: the POU domain by itself shows some activity with a promoter where the octamer sequence is located very close to the TATA box, but no activity with another promoter construction where the octamer sequence is located further upstream. The two promoters also respond differently to the deletion of the glutamine-rich stretch important for transcriptional activation. From these experiments we consider it likely that the natural octamer factor variants can selectively activate the different naturally occurring octamer-containing promoters.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.