ArticlePloS one2012
Role of actin dependent nuclear deformation in regulating early gene expression.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
39 citing papers in PubMed.
- Stiff matrix-induced KRTAP2-3 expression suppresses ciliogenesis via actin tension-driven chromatin remodeling.Cell death & disease · 2026Article
- Integrin-Mediated Mechanosensing of Modeled Lymph Node Microenvironment Promotes T Cell Activation via Nuclear Deformation.Research (Washington, D.C.) · 2026Article
- Mechanical forces in the tumor microenvironment: roles, pathways, and therapeutic approaches.Journal of translational medicine · 2025Review
- Three-dimensional isotropic imaging of live suspension cells enabled by droplet microvortices.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Unsupervised representation learning of chromatin images identifies changes in cell state and tissue organization in DCIS.Nature communications · 2024Article
- Natural Killer Cell Mechanosensing in Solid Tumors.Bioengineering (Basel, Switzerland) · 2024Review
- Imaging and AI based chromatin biomarkers for diagnosis and therapy evaluation from liquid biopsies.NPJ precision oncology · 2023Article
- Agarose-based 3D Cell Confinement Assay to Study Nuclear Mechanobiology.Current protocols · 2023Article
- The application of mechanical load onto mouse tendons by magnetic restraining represses Mmp-3 expression.BMC research notes · 2023Article
- Article
- Fluid shear stress promotes periodontal ligament cells proliferation via p38-AMOT-YAP.Cellular and molecular life sciences : CMLS · 2022Article
- 3D analyses reveal T cells with activated nuclear features in T-cell/histiocyte-rich large B-cell lymphoma.Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc · 2022Article
- Mechanics and functional consequences of nuclear deformations.Nature reviews. Molecular cell biology · 2022Review
- The Elephant in the Cell: Nuclear Mechanics and Mechanobiology.Journal of biomechanical engineering · 2022Review
- Appreciating the role of cell shape changes in the mechanobiology of epithelial tissues.Biophysics reviews · 2022Review
- The 3D Genome: From Structure to Function.International journal of molecular sciences · 2021Review
- Ezh2 harnesses the intranuclear actin cytoskeleton to remodel chromatin in differentiating Th cells.iScience · 2021Article
- Interrogating RNA and protein spatial subcellular distribution in smFISH data with DypFISH.Cell reports methods · 2021Article
- Modelling Nuclear Morphology and Shape Transformation: A Review.Membranes · 2021Review
- Biophysical interactions between components of the tumor microenvironment promote metastasis.Biophysical reviews · 2021Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The nucleus of a living cell is constantly undergoing changes in shape and size as a result of various mechanical forces in physiology. These changes correlate with alterations in gene expression, however it is unclear whether nuclear deformation alone is sufficient to elicit these alterations. We used T-cell activation as a model system to test the coupling between nuclear deformation (elongation) and gene expression. Naïve T-cell activation with surrogate antigens resulted in actin dependent nuclear elongation. This was accompanied with Erk and NF-κB signaling to the nucleus to induce CD69 expression. Importantly, inhibiting actin polymerization abolished both nuclear elongation and CD69 expression, while inhibiting Erk, NF-κB or microtubule depolymerization only abolished expression but not elongation. Immobilization of antigen-coated beads, under conditions where actin polymerization was inhibited, rescued both nuclear elongation and CD69 expression. In addition, fibroblast cells plated on fibronectin micropatterns of different sizes showed correlation between nuclear shape index and tenascin C expression. Upon inhibiting the signaling intermediate Erk, tenascin C expression was down regulated although the nuclear shape index remained unaltered. Our results highlight the importance of specific signaling intermediates accompanied with nuclear deformation in the modulation of cellular genomic programs.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.