ArticlePloS one2012
Domestic mite antigens in floor and airborne dust at workplaces in comparison to living areas: a new immunoassay to assess personal airborne allergen exposure.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed, 38 citations in OpenAlex.
- Review
- Legumes in bread baking: A hidden risk for an IgE-mediated inhalant allergy.Allergologie select · 2025Article
- Exposure levels of animal allergens, endotoxin, and β-(1,3)-glucan on a university campus of veterinary medicine.PloS one · 2023Article
- Comparing the concentration levels of allergens and endotoxins in employees' homes and offices.International archives of occupational and environmental health · 2022Article
- Animal Allergens, Endotoxin, and β-(1,3)-Glucan in Small Animal Practices: Exposure Levels at Work and in Homes of Veterinary Staff.Annals of work exposures and health · 2022Article
- Use of nasal filters for allergen exposure measurements in veterinary practices.Environmental and occupational health practice · 2022Article
- Clinical significance of dust mite allergens.Molecular biology reports · 2020Review
- The Role of Dust Mites in Allergy.Clinical reviews in allergy & immunology · 2019Review
- International consensus (ICON) on: clinical consequences of mite hypersensitivity, a global problem.The World Allergy Organization journal · 2017Article
- ELISA in the multiplex era: potentials and pitfalls.Proteomics. Clinical applications · 2015Review
- When mites attack: domestic mites are not just allergens.Parasites & vectors · 2014Review
- Article
- Mites and other indoor allergens - from exposure to sensitization and treatment.Allergo journal internationalReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectivesAllergens produced by domestic mites (DM) are among the most common allergic sensitizers and risk factors for asthma. To compare exposure levels between workplaces and living areas a new assay able to measure airborne DM antigen concentrations was developed.
methodsAt workplaces and in living areas, 213 floor dust samples and 92 personal inhalable dust samples were collected. For sensitive quantification of DM antigens, a new enzyme immunoassay (EIA) based on polyclonal antibodies to Dermatophagoides farinae extract was developed. Reactivity of five house dust mite and four storage mite species was tested. All dust samples were tested with the new EIA and with the Der f 1 and Der p 1-EIAs (Indoor Biotechnologies, UK) which detect major allergens from D. farinae and D. pteronyssinus by monoclonal antibodies. Samples below the detection limit in the DM-EIA were retested in an assay variant with a fluorogenic substrate (DM-FEIA).
resultsThe newly developed DM-EIA detects antigens from all nine tested domestic mite species. It has a lower detection limit of 200 pg/ml of D.farinae protein, compared to 50 pg/ml for the DM-FEIA. DM antigens were detected by DM-EIA/FEIA in all floor dust and 80 (87%) of airborne samples. Der f 1 was found in 133 (62%) floor dust and in only 6 airborne samples, Der p 1 was found in 70 (33%) of floor samples and in one airborne sample. Der f 1 and DM concentrations were highly correlated. DM-antigens were significantly higher in inhalable airborne samples from textile recycling, bed feather filling, feed production, grain storage and cattle stables in comparison to living areas.
conclusionsA new sensitive EIA directed at DM antigens was developed. DM antigen quantities were well correlated to Der f 1 values and were measurable in the majority (87%) of airborne dust samples. Some workplaces had significantly higher DM antigen concentrations than living areas.
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