ArticlePloS one2012
Rapid and serial quantification of adhesion forces of yeast and Mammalian cells.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 130 citations in OpenAlex.
- Article
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- Characterization of a unique attachment organelle: Single-cell force spectroscopy ofNanoscale · 2024Article
- Microfluidic device combining hydrodynamic and dielectrophoretic trapping for the controlled contact between single micro-sized objects and application to adhesion assays.Lab on a chip · 2023Article
- Recent advances in single-cell subcellular sampling.Chemical communications (Cambridge, England) · 2023Review
- 4D Force Detection of Cell Adhesion and Contractility.Nano letters · 2023Article
- Extending applications of AFM to fluidic AFM in single living cell studies.Journal of cellular physiology · 2022Review
- Population distributions of single-cell adhesion parameters during the cell cycle from high-throughput robotic fluidic force microscopy.Scientific reports · 2022Article
- Population distributions of single-cell adhesion parameters during the cell cycle from high-throughput robotic fluidic force microscopy.Scientific reports · 2022Article
- Micropipette-based biomechanical nanotools on living cells.European biophysics journal : EBJ · 2022Review
- Microfabricated cantilevers for parallelized cell-cell adhesion measurements.European biophysics journal : EBJ · 2022Article
- Injection into and extraction from single fungal cells.Communications biology · 2022Article
- Cell-Cell Mating Interactions: Overview and Potential of Single-Cell Force Spectroscopy.International journal of molecular sciences · 2022Review
- Quantifying molecular- to cellular-level forces in living cells.Journal of physics D: Applied physics · 2021Article
- Quantification of the Adhesion Strength ofMicroorganisms · 2021Article
- From the first touch to biofilm establishment by the human pathogen Candida glabrata: a genome-wide to nanoscale view.Communications biology · 2021Article
- Differential cellular responses to adhesive interactions with galectin-8- and fibronectin-coated substrates.Journal of cell science · 2021Article
- Single-cell fluidic force microscopy reveals stress-dependent molecular interactions in yeast mating.Communications biology · 2021Article
- Article
- Sensing and Modelling Mechanical Response in Large Deformation Indentation of Adherent Cell Using Atomic Force Microscopy.Sensors (Basel, Switzerland) · 2020Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
Cell adhesion to surfaces represents the basis for niche colonization and survival. Here we establish serial quantification of adhesion forces of different cell types using a single probe. The pace of single-cell force-spectroscopy was accelerated to up to 200 yeast and 20 mammalian cells per probe when replacing the conventional cell trapping cantilever chemistry of atomic force microscopy by underpressure immobilization with fluidic force microscopy (FluidFM). In consequence, statistically relevant data could be recorded in a rapid manner, the spectrum of examinable cells was enlarged, and the cell physiology preserved until approached for force spectroscopy. Adhesion forces of Candida albicans increased from below 4 up to 16 nN at 37°C on hydrophobic surfaces, whereas a Δhgc1-mutant showed forces consistently below 4 nN. Monitoring adhesion of mammalian cells revealed mean adhesion forces of 600 nN of HeLa cells on fibronectin and were one order of magnitude higher than those observed for HEK cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.