ArticlePloS one2012
Reconstruction and evaluation of the synthetic bacterial MEP pathway in Saccharomyces cerevisiae.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed, 73 citations in OpenAlex.
- Metabolic engineering strategies: utilizing different microbial strains and advanced technologies for the synthesis of high-valued terpenoids.Antonie van Leeuwenhoek · 2026Review
- Heterologous iron-sulfur cluster biogenesis and delivery for cytosolic isobutanol and isopentanol production inbioRxiv : the preprint server for biology · 2026Article
- Harnessing evolution: leveraging bacterial isoprenoid pathway diversity toward improved bioengineering strategies.Journal of bacteriology · 2026Review
- Production of the terpenoid-based biofuel α-bisabolene by metabolically engineered Corynebacterium glutamicum.World journal of microbiology & biotechnology · 2025Article
- Orthogonal replication with optogenetic selection evolves yeast JEN1 into a mevalonate transporter.Molecular systems biology · 2025Article
- Matrix regulation: a plug-and-tune method for combinatorial regulation in Saccharomyces cerevisiae.Nature communications · 2025Article
- The methylerythritol phosphate pathway as an oxidative stress sense and response system.Nature communications · 2024Review
- EngineeringEngineering microbiology · 2024Review
- The non-mevalonate pathway requires a delicate balance of intermediates to maximize terpene production.Applied microbiology and biotechnology · 2024Article
- Apicoplast-Resident Processes: Exploiting the Chink in the Armour ofAdvances in pharmacological and pharmaceutical sciences · 2024Review
- Advances in regulating vitamin KWorld journal of microbiology & biotechnology · 2023Review
- A highly efficient transcriptome-based biosynthesis of non-ethanol chemicals in Crabtree negative Saccharomyces cerevisiae.Biotechnology for biofuels and bioproducts · 2023Article
- Engineering a universal and efficient platform for terpenoid synthesis in yeast.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Metabolic recycling of storage lipids promotes squalene biosynthesis in yeast.Biotechnology for biofuels and bioproducts · 2022Article
- Apicoplast Journey and Its Essentiality as a Compartment for Malaria Parasite Survival.Frontiers in cellular and infection microbiology · 2022Article
- Towards a Synthetic Biology Toolset for Metallocluster Enzymes in Biosynthetic Pathways: What We Know and What We Need.Molecules (Basel, Switzerland) · 2021Review
- Article
- Functional replacement of isoprenoid pathways in Rhodobacter sphaeroides.Microbial biotechnology · 2020Article
- Modular engineering for microbial production of carotenoids.Metabolic engineering communications · 2020Review
- Investigation of the methylerythritol 4-phosphate pathway for microbial terpenoid production through metabolic control analysis.Microbial cell factories · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Isoprenoids, which are a large group of natural and chemical compounds with a variety of applications as e.g. fragrances, pharmaceuticals and potential biofuels, are produced via two different metabolic pathways, the mevalonate (MVA) pathway and the 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway. Here, we attempted to replace the endogenous MVA pathway in Saccharomyces cerevisiae by a synthetic bacterial MEP pathway integrated into the genome to benefit from its superior properties in terms of energy consumption and productivity at defined growth conditions. It was shown that the growth of a MVA pathway deficient S. cerevisiae strain could not be restored by the heterologous MEP pathway even when accompanied by the co-expression of genes erpA, hISCA1 and CpIscA involved in the Fe-S trafficking routes leading to maturation of IspG and IspH and E. coli genes fldA and fpr encoding flavodoxin and flavodoxin reductase believed to be responsible for electron transfer to IspG and IspH.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.