ArticlePloS one2012
Enhancing endosomal escape of transduced proteins by photochemical internalisation.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 42 citations in OpenAlex.
- Achieving Endo/Lysosomal Escape Using Smart Nanosystems for Efficient Cellular Delivery.Molecules (Basel, Switzerland) · 2024Review
- A Strategy for Gene Knockdown in Dinoflagellates.Microorganisms · 2022Article
- Targeting the Inside of Cells with Biologicals: Chemicals as a Delivery Strategy.BioDrugs : clinical immunotherapeutics, biopharmaceuticals and gene therapy · 2021Review
- Cell cycle dependence of apoptosis photo-triggered using peptide-photosensitizer conjugate.Scientific reports · 2020Article
- Restoring functional neurofibromin by protein transduction.Scientific reports · 2018Article
- Effective photo-enhancement of cellular activity of fluorophore-octaarginine antisense PNA conjugates correlates with singlet oxygen formation, endosomal escape and chromophore lipophilicity.Scientific reports · 2018Article
- Irreversible Protein Labeling by Paal-Knorr Conjugation.Chembiochem : a European journal of chemical biology · 2017Article
- Intracellular delivery of proteins by nanocarriers.Nanomedicine (London, England) · 2017Review
- Cellular uptake and anti-tumor activity of gemcitabine conjugated with new amphiphilic cell penetrating peptides.EXCLI journal · 2017Article
- Nanotechnology for protein delivery: Overview and perspectives.Journal of controlled release : official journal of the Controlled Release Society · 2016Review
- Effect of poly-glutamate on uptake efficiency and cytotoxicity of cell penetrating peptides.IET nanobiotechnology · 2016Article
- Targeting polyIC to EGFR over-expressing cells using a dsRNA binding protein domain tethered to EGF.PloS one · 2016Article
- Near-infrared light activated delivery platform for cancer therapy.Advances in colloid and interface science · 2015Review
- Effects of employment of distinct strategies to capture antibody on antibody delivery into cultured cells.Molecular and cellular biochemistry · 2015Article
- Promises and pitfalls of intracellular delivery of proteins.Bioconjugate chemistry · 2014Review
- Fabrication of multiresponsive bioactive nanocapsules through orthogonal self-assembly.Angewandte Chemie (International ed. in English) · 2014Article
- Targeted genome editing by lentiviral protein transduction of zinc-finger and TAL-effector nucleases.eLife · 2014Article
- DNA transposition by protein transduction of the piggyBac transposase from lentiviral Gag precursors.Nucleic acids research · 2014Article
- Driving DNA transposition by lentiviral protein transduction.Mobile genetic elements · 2014Article
- Protein transfection study using multicellular tumor spheroids of human hepatoma Huh-7 cells.PloS one · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Induced internalisation of functional proteins into cultured cells has become an important aspect in a rising number of in vitro and in vivo assays. The endo-lysosomal entrapment of the transduced proteins remains the major problem in all transduction protocols. In this study we compared the efficiency, cytotoxicity and protein targeting of different commercially available transduction reagents by transducing a well-studied fluorescently labelled protein (Atto488-bovine serum albumin) into cultured human sarcoma cells. The amount of internalised protein and toxicity differed between the different reagents, but the percentage of transduced cells was consistently high. Furthermore, in all protocols the signals of the transduced Atto488-BSA were predominantly punctual consistent with an endosomal localisation. To overcome the endosomal entrapment, the transduction protocols were combined with a photochemical internalisation (PCI) treatment. Using this combination revealed that an endosomal disruption is highly effective in cell penetrating peptide (CPP) mediated transduction, whereas lipid-mediated transductions lead to a lower signal spreading throughout the cytosol. No change in the signal distribution could be achieved in treatments using non-lipid polymers as a transduction reagent. Therefore, the combination of protein transduction protocols based on CPPs with the endosomolytic treatment PCI can facilitate protein transduction experiments in vitro.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.