Trial reportPloS one2012
Modulation of protein fermentation does not affect fecal water toxicity: a randomized cross-over study in healthy subjects.
Trial report in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT01280513 (Influence of a Protein Diet on the Colonic Metabolism and Phosphorus Housekeeping in Healthy Volunteers.), which is not on this map. Cited by 31 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Influence of a Protein Diet on the Colonic Metabolism and Phosphorus Housekeeping in Healthy Volunteers.
Who cites it
31 citing papers in PubMed, 1 synthesis or guideline pooled it, 72 citations in OpenAlex.
- Meat Consumption and Gut Microbiota: a Scoping Review of Literature and Systematic Review of Randomized Controlled Trials in Adults.Advances in nutrition (Bethesda, Md.) · 2023Pooled it
- Protein supplementation during an energy-restricted diet induces visceral fat loss and gut microbiota amino acid metabolism activation: a randomized trial.Scientific reports · 2021Trial
- A period of 10 weeks of increased protein consumption does not alter faecal microbiota or volatile metabolites in healthy older men: a randomised controlled trial.Journal of nutritional science · 2020Trial
- Acute stressor alters inter-species microbial competition for resistant starch-supplemented medium.Gut microbes · 2019Trial
- Associations between the gut microbiota and host responses to high altitude.American journal of physiology. Gastrointestinal and liver physiology · 2018Trial
- The Influence of Dietary Protein Intake on Mammalian Tryptophan and Phenolic Metabolites.PloS one · 2015Trial
- Amino Acid-Derived Bacterial Metabolites in the Colorectal Luminal Fluid: Effects on Microbial Communication, Metabolism, Physiology, and Growth.Microorganisms · 2023Review
- Genome-centric investigation of the potential succession pattern in gut microbiota and altered functions under high-protein diet.Current research in food science · 2023Article
- Antiproliferative activity of antimicrobial peptides and bioactive compounds from the mangroveFrontiers in microbiology · 2023Article
- The Microbiota and the Gut-Brain Axis in Controlling Food Intake and Energy Homeostasis.International journal of molecular sciences · 2021Review
- Gut Microbiota Metabolism and Interaction with Food Components.International journal of molecular sciences · 2020Review
- Establishing What Constitutes a Healthy Human Gut Microbiome: State of the Science, Regulatory Considerations, and Future Directions.The Journal of nutrition · 2019Review
- The Role of the Gut Microbiota in Colorectal Cancer Causation.International journal of molecular sciences · 2019Review
- Observational
- Review
- Modelling the role of microbial p-cresol in colorectal genotoxicity.Gut microbes · 2019Article
- Metabolite quantification of faecal extracts from colorectal cancer patients and healthy controls.Oncotarget · 2018Article
- Article
- Effects of Psychological, Environmental and Physical Stressors on the Gut Microbiota.Frontiers in microbiology · 2018Review
- Dietary Protein and Amino Acid Supplementation in Inflammatory Bowel Disease Course: What Impact on the Colonic Mucosa?Nutrients · 2017Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveProtein fermentation results in production of metabolites such as ammonia, amines and indolic, phenolic and sulfur-containing compounds. In vitro studies suggest that these metabolites might be toxic. However, human and animal studies do not consistently support these findings. We modified protein fermentation in healthy subjects to assess the effects on colonic metabolism and parameters of gut health, and to identify metabolites associated with toxicity.
designAfter a 2-week run-in period with normal protein intake (NP), 20 healthy subjects followed an isocaloric high protein (HP) and low protein (LP) diet for 2 weeks in a cross-over design. Protein fermentation was estimated from urinary p-cresol excretion. Fecal metabolite profiles were analyzed using GC-MS and compared using cluster analysis. DGGE was used to analyze microbiota composition. Fecal water genotoxicity and cytotoxicity were determined using the Comet assay and the WST-1-assay, respectively, and were related to the metabolite profiles.
resultsDietary protein intake was significantly higher during the HP diet compared to the NP and LP diet. Urinary p-cresol excretion correlated positively with protein intake. Fecal water cytotoxicity correlated negatively with protein fermentation, while fecal water genotoxicity was not correlated with protein fermentation. Heptanal, 3-methyl-2-butanone, dimethyl disulfide and 2-propenyl ester of acetic acid are associated with genotoxicity and indole, 1-octanol, heptanal, 2,4-dithiapentane, allyl-isothiocyanate, 1-methyl-4-(1-methylethenyl)-benzene, propionic acid, octanoic acid, nonanoic acid and decanoic acid with cytotoxicity.
conclusionThis study does not support a role of protein fermentation in gut toxicity. The identified metabolites can provide new insight into colonic health.
trial registrationClinicalTrial.gov NCT01280513.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.