ArticlePloS one2012
Development of a real-time PCR for identification of brachyspira species in human colonic biopsies.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 1 synthesis or guideline pooled it, 29 citations in OpenAlex.
- Human intestinal spirochetosis, irritable bowel syndrome, and colonic polyps: A systematic review and meta-analysis.Journal of gastroenterology and hepatology · 2022Pooled it
- Prevalence of intestinal spirochaetosis in serrated polyposis syndrome compared with other colorectal polyp conditions: a case-control study in Japan.BMJ open gastroenterology · 2026Article
- Research note: occurrence, molecular characterization, and clinical associations of avian intestinal spirochetosis in laying hens in India.Poultry science · 2026Article
- Human Intestinal Spirochetosis Shows Higher Positivity in Sessile Serrated Lesions Than in Tubular Adenomas With Preferential Colonization of Absorptive Epithelium.Pathology international · 2026Article
- Identification of Terminal βGlcNAc onThe journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2024Article
- Association betweenGut · 2021Article
- Isolates from Colonic Spirochetosis in Humans Show High Genomic Divergence and Potential Pathogenic Features but Are Not Detected Using Standard Primers for the Human Microbiota.Journal of bacteriology · 2019Article
- Hiding in Plain Sight: Colonic Spirochetosis in Humans.Journal of bacteriology · 2019Article
- Polymerase chain reaction assay targetingVeterinary research forum : an international quarterly journal · 2019Article
- Treponema pallidum Immunohistochemistry is positive in human intestinal Spirochetosis.Diagnostic pathology · 2018Article
- The Spirochete Brachyspira pilosicoli, Enteric Pathogen of Animals and Humans.Clinical microbiology reviews · 2018Review
- Current and past strategies for bacterial culture in clinical microbiology.Clinical microbiology reviews · 2015Review
- Intestinal spirochaetes (genus Brachyspira) colonise wild birds in the southern Atlantic region and Antarctica.Infection ecology & epidemiology · 2015Article
- The first 1000 cultured species of the human gastrointestinal microbiota.FEMS microbiology reviews · 2014Review
- Appendiceal spirochaetosis in children.Gut pathogens · 2013Article
- Brachyspira species and gastroenteritis in humans.Journal of clinical microbiology · 2013Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundBrachyspira species are fastidious anaerobic microorganisms, that infect the colon of various animals. The genus contains both important pathogens of livestock as well as commensals. Two species are known to infect humans: B. aalborgi and B. pilosicoli. There is some evidence suggesting that the veterinary pathogenic B. pilosicoli is a potential zoonotic agent, however, since diagnosis in humans is based on histopathology of colon biopsies, species identification is not routinely performed in human materials.
methodsThe study population comprised 57 patients with microscopic evidence of Brachyspira infection and 26 patients with no histopathological evidence of Brachyspira infection. Concomitant faecal samples were available from three infected patients. Based on publically available 16S rDNA gene sequences of all Brachyspira species, species-specific primer sets were designed. DNA was extracted and tested by real-time PCR and 16S rDNA was sequenced.
resultsSensitivity and specificity for identification of Brachyspira species in colon biopsies was 100% and 87.7% respectively. Sequencing revealed B. pilosicoli in 15.4% of patients, B. aalborgi in 76.9% and a third species, tentatively named "Brachyspira hominis", in 26.2%. Ten patients (12.3%) had a double and two (3.1%) a triple infection. The presence of Brachyspira pilosicoli was significantly associated with inflammatory changes in the colon-biopsy (p=0.028).
conclusionsThis newly designed PCR allows for sub-differentiation of Brachyspira species in patient material and thus allows large-scaled surveillance studies to elucidate the pathogenicity of human Brachyspira infections. One-third of affected patients appeared to be infected with a novel species.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.