ArticlePloS one2012
Analysis of mitochondrial function and localisation during human embryonic stem cell differentiation in vitro.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed, 48 citations in OpenAlex.
- TGFβ-dependent upregulation of OCIAD2 is essential for epithelial-to-mesenchymal transition during mesendoderm differentiation.Stem cell reports · 2026Article
- Mitochondrial development and remodeling occur at pancreatic progenitor stage during induction of stem cell-derived islet organoids.Frontiers in endocrinology · 2026Article
- Viral vaccines promote endoplasmic reticulum stress-induced unfolding protein response in teleost erythrocytes.European journal of cell biology · 2025Article
- Advances in human pluripotent stem cell reporter systems.iScience · 2024Review
- Matrix stiffening promotes perinuclear clustering of mitochondria.Molecular biology of the cell · 2024Article
- Deciphering the Heterogeneity of Mitochondrial Functions During Hematopoietic Lineage Differentiation.Stem cell reviews and reports · 2022Article
- Bone morphogenetic protein 4 rescues the bone regenerative potential of old muscle-derived stem cells via regulation of cell cycle inhibitors.Stem cell research & therapy · 2022Article
- High Glycolytic Activity Enhances Stem Cell Reprogramming of Fahd1-KO Mouse Embryonic Fibroblasts.Cells · 2021Article
- Development-related mitochondrial properties of retinal pigment epithelium cells derived from hEROs.International journal of ophthalmology · 2021Article
- Biochemical re-programming of human dermal stem cells to neurons by increasing mitochondrial membrane potential.Cell death and differentiation · 2019Article
- Dysfunction of Mesenchymal Stem Cells Isolated from Metabolic Syndrome and Type 2 Diabetic Patients as Result of Oxidative Stress and Autophagy may Limit Their Potential Therapeutic Use.Stem cell reviews and reports · 2018Review
- The TDH-GCN5L1-Fbxo15-KBP axis limits mitochondrial biogenesis in mouse embryonic stem cells.Nature cell biology · 2017Article
- Cardiomyocyte Differentiation Promotes Cell Survival During Nicotinamide Phosphoribosyltransferase Inhibition Through Increased Maintenance of Cellular Energy Stores.Stem cells translational medicine · 2017Article
- Equine metabolic syndrome impairs adipose stem cells osteogenic differentiation by predominance of autophagy over selective mitophagy.Journal of cellular and molecular medicine · 2016Article
- Therapeutic interactions between mesenchymal stem cells for healing medication-related osteonecrosis of the jaw.Stem cell research & therapy · 2016Article
- High-throughput detection and quantification of mitochondrial fusion through imaging flow cytometry.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2016Article
- Long range physical cell-to-cell signalling via mitochondria inside membrane nanotubes: a hypothesis.Theoretical biology & medical modelling · 2016Review
- Murine Mesenchymal Stem Cell Commitment to Differentiation Is Regulated by Mitochondrial Dynamics.Stem cells (Dayton, Ohio) · 2016Article
- Human pluripotent stem cells: Prospects and challenges as a source of cardiomyocytes for in vitro modeling and cell-based cardiac repair.Advanced drug delivery reviews · 2016Review
- Connecting Mitochondria, Metabolism, and Stem Cell Fate.Stem cells and development · 2015Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human embryonic stem cell (hESC) derivatives show promise as viable cell therapy options for multiple disorders in different tissues. Recent advances in stem cell biology have lead to the reliable production and detailed molecular characterisation of a range of cell-types. However, the role of mitochondria during differentiation has yet to be fully elucidated. Mitochondria mediate a cells response to altered energy requirements (e.g. cardiomyocyte contraction) and, as such, the mitochondrial phenotype is likely to change during the dynamic process of hESC differentiation. We demonstrate that manipulating mitochondrial biogenesis alters mesendoderm commitment. To investigate mitochondrial localisation during early lineage specification of hESCs we developed a mitochondrial reporter line, KMEL2, in which sequences encoding the green fluorescent protein (GFP) are targeted to the mitochondria. Differentiation of KMEL2 lines into the three germ layers showed that the mitochondria in these differentiated progeny are GFP positive. Therefore, KMEL2 hESCs facilitate the study of mitochondria in a range of cell types and, importantly, permit real-time analysis of mitochondria via the GFP tag.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.