ArticlePloS one2012
Influence of the internalization pathway on the efficacy of siRNA delivery by cationic fluorescent nanodiamonds in the Ewing sarcoma cell model.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 66 citations in OpenAlex.
- Nanodiamonds: Next generation nano-theranostics for cancer therapy.Cancer letters · 2024Review
- Intracellular Relaxometry, Challenges, and Future Directions.ACS central science · 2022Review
- Functionalized Fluorescent Nanodiamonds for Simultaneous Drug Delivery and Quantum Sensing in HeLa Cells.ACS applied materials & interfaces · 2022Article
- Carbon-based Nanomaterials for Delivery of Small RNA Molecules: A Focus on Potential Cancer Treatment Applications.Pharmaceutical nanotechnology · 2022Review
- Nanodiamonds with powerful ability for drug delivery and biomedical applications: Recent updates on in vivo study and patents.Journal of pharmaceutical analysis · 2020Review
- Knockdown of microRNA-135b in Mammary Carcinoma by Targeted Nanodiamonds: Potentials and Pitfalls of In Vivo Applications.Nanomaterials (Basel, Switzerland) · 2019Article
- The origins and evolution of macropinocytosis.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2019Review
- Fluorescent Nanodiamond Applications for Cellular Process Sensing and Cell Tracking.Micromachines · 2018Review
- The Response of HeLa Cells to Fluorescent NanoDiamond Uptake.Sensors (Basel, Switzerland) · 2018Article
- Nanodiamonds facilitate killing of intracellular uropathogenic E. coli in an in vitro model of urinary tract infection pathogenesis.PloS one · 2018Article
- Cellular uptake pathways of sepiolite nanofibers and DNA transfection improvement.Scientific reports · 2017Article
- Lysine-functionalized nanodiamonds as gene carriers: development of stable colloidal dispersion for in vitro cellular uptake studies and siRNA delivery application.International journal of nanomedicine · 2016Article
- Docetaxel-carboxymethylcellulose nanoparticles target cells via a SPARC and albumin dependent mechanism.Biomaterials · 2015Article
- Nanodiamonds: The intersection of nanotechnology, drug development, and personalized medicine.Science advances · 2015Review
- Fluorescent nanodiamonds as a relevant tag for the assessment of alum adjuvant particle biodisposition.BMC medicine · 2015Article
- Twenty Years on: What Do We Really Know about Ewing Sarcoma and What Is the Path Forward?Critical reviews in oncogenesis · 2015Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Small interfering RNAs (siRNAs) are powerful tools commonly used for the specific inhibition of gene expression. However, vectorization is required to facilitate cell penetration and to prevent siRNA degradation by nucleases. We have shown that diamond nanocrystals coated with cationic polymer can be used to carry siRNAs into Ewing sarcoma cells, in which they remain traceable over long periods, due to their intrinsic stable fluorescence. We tested two cationic polymers, polyallylamine and polyethylenimine. The release of siRNA, accompanied by Ewing sarcoma EWS-Fli1 oncogene silencing, was observed only with polyethylenimine. We investigated cell penetration and found that the underlying mechanisms accounted for these differences in behavior. Using drugs selectively inhibiting particular pathways and a combination of fluorescence and electronic microscopy, we showed that siRNA gene silencing occurred only if the siRNA:cationic nanodiamond complex followed the macropinocytosis route. These results have potential implications for the design of efficient drug-delivery vectors.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.