ArticlePloS one2012
Quantitative, non-disruptive monitoring of transcription in single cells with a broad-host range GFP-luxCDABE dual reporter system.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Structural analysis of the NifL-NifA complex reveals the molecular basis of anti-activation of nitrogen fixation gene expression in Azotobacter vinelandii.The FEBS journal · 2026Article
- Improving a Tn7-based luciferase reporter system for promoter activity studies.Microbiology (Reading, England) · 2026Article
- From macro to micro: a combined bioluminescence-fluorescence approach to monitor bacterial localization.Environmental microbiology · 2021Article
- Exploiting geometric similarity for statistical quantification of fluorescence spatial patterns in bacterial colonies.BMC bioinformatics · 2020Article
- Construction of a RFP-lacZα bicistronic reporter system and its application in lead biosensing.PloS one · 2020Article
- Synthetic metabolic computation in a bioluminescence-sensing system.Nucleic acids research · 2019Article
- Thermoregulation of Prodigiosin Biosynthesis byPolish journal of microbiology · 2019Article
- Blood meal-induced inhibition of vector-borne disease by transgenic microbiota.Nature communications · 2018Article
- Article
- Calibrating Transcriptional Activity Using Constitutive Synthetic Promoters in Mutants for Global Regulators inInternational journal of genomics · 2018Article
- Zinc-Induced Transposition of Insertion Sequence Elements Contributes to Increased Adaptability of Cupriavidus metallidurans.Frontiers in microbiology · 2016Article
- Pseudomonas 2.0: genetic upgrading of P. putida KT2440 as an enhanced host for heterologous gene expression.Microbial cell factories · 2014Article
- Broad host range vectors for expression of proteins with (Twin-) Strep-tag, His-tag and engineered, export optimized yellow fluorescent protein.Microbial cell factories · 2013Article
- Synthetic and minimalist vectors for Agrobacterium tumefaciens-mediated transformation of fungi.Genetics and molecular biologyArticle
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A dual promoter probe system based on a tandem bi-cistronic GFP-luxCDABE reporter cassette is described and implemented. This system is assembled in two synthetic, modular, broad-host range plasmids based on pBBR1 and RK2 origins of replication, allowing its utilization in an extensive number of gram-negative bacteria. We analyze the performance of this dual cassette in two hosts, Escherichia coli and Pseudomonas putida, by examining the induction properties of the lacI(q)-Ptrc expression system in the first host and the Pb promoter of the benzoate degradation pathway in the second host. By quantifying the bioluminescence signal produced through the expression of the lux genes, we explore the dynamic range of induction for the two systems (Ptrc-based and Pb-based) in response to the two inducers. In addition, by quantifying the fluorescence signals produced by GFP expression, we were able to monitor the single-cell expression profile and to explore stochasticity of the same two promoters by flow cytometry. The results provided here demonstrate the power of the dual GFP-luxCDABE cassette as a new, single-step tool to assess promoter properties at both the population and single-cell levels in gram-negative bacteria.
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