ArticlePloS one2012
Monitoring intracellular calcium ion dynamics in hair cell populations with Fluo-4 AM.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 44 citations in OpenAlex.
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- Enteric Nervous System: Identification of a Novel Neuronal Sensory Network in the Duodenal Epithelium.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2023Article
- Regulation of membrane homeostasis by TMC1 mechanoelectrical transduction channels is essential for hearing.Science advances · 2022Article
- Calcium released by osteoclastic resorption stimulates autocrine/paracrine activities in local osteogenic cells to promote coupled bone formation.American journal of physiology. Cell physiology · 2022Article
- Ursolic acid protects against cisplatin‑induced ototoxicity by inhibiting oxidative stress and TRPV1‑mediated Ca2+‑signaling.International journal of molecular medicine · 2020Article
- ATP11B deficiency leads to impairment of hippocampal synaptic plasticity.Journal of molecular cell biology · 2019Article
- Rationally Designed α-Conotoxin Analogues Maintained Analgesia Activity and Weakened Side Effects.Molecules (Basel, Switzerland) · 2019Article
- Spontaneous and Acetylcholine Evoked Calcium Transients in the Developing Mouse Utricle.Frontiers in cellular neuroscience · 2019Article
- Protease activated receptor 2 mediates tryptase-induced cell migration through MYO10 in colorectal cancer.American journal of cancer research · 2019Article
- Melatonin Affects Mitochondrial Fission/Fusion Dynamics in the Diabetic Retina.Journal of diabetes research · 2019Article
- In Vivo Calcium Imaging of Lateral-line Hair Cells in Larval Zebrafish.Journal of visualized experiments : JoVE · 2018Article
- Structural relationship between the putative hair cell mechanotransduction channel TMC1 and TMEM16 proteins.eLife · 2018Article
- A Model for Link Pruning to Establish Correctly Polarized and Oriented Tip Links in Hair Bundles.Biophysical journal · 2017Article
- A High-Throughput Automated Microfluidic Platform for Calcium Imaging of Taste Sensing.Molecules (Basel, Switzerland) · 2016Article
- Combined Cytological and Transcriptomic Analysis Reveals a Nitric Oxide Signaling Pathway Involved in Cold-Inhibited Camellia sinensis Pollen Tube Growth.Frontiers in plant science · 2016Article
- Immunohistochemical and calcium imaging methods in wholemount rat retina.Journal of visualized experiments : JoVE · 2014Article
- Correlation of actin crosslinker and capper expression levels with stereocilia growth phases.Molecular & cellular proteomics : MCP · 2014Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
We optimized Fluo-4 AM loading of chicken cochlea to report hair-bundle Ca(2+) signals in populations of hair cells. The bundle Ca(2+) signal reported the physiological state of the bundle and cell; extruding cells had very high bundle Fluo-4 fluorescence, cells with intact bundles and tip links had intermediate fluorescence, and damaged cells with broken tip links had low fluorescence. Moreover, Fluo-4 fluorescence in the bundle correlated with Ca(2+) entry through transduction channels; mechanically activating transduction channels increased the Fluo-4 signal, while breaking tip links with Ca(2+) chelators or blocking Ca(2+) entry through transduction channels each caused bundle and cell-body Fluo-4 fluorescence to decrease. These results show that when tip links break, bundle and soma Ca(2+) decrease, which could serve to stimulate the hair cell's tip-link regeneration process. Measurement of bundle Ca(2+) with Fluo-4 AM is therefore a simple method for assessing mechanotransduction in hair cells and permits an increased understanding of the interplay of tip links, transduction channels, and Ca(2+) signaling in the hair cell.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.