ArticlePloS one2012
A mechanistic model of early FcεRI signaling: lipid rafts and the question of protection from dephosphorylation.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 25 citations in OpenAlex.
- Direct quantification of ligand-induced lipid and protein microdomains with distinctive signaling properties.ChemSystemsChem · 2022Article
- Transbilayer Coupling of Lipids in Cells Investigated by Imaging Fluorescence Correlation Spectroscopy.The journal of physical chemistry. B · 2022Article
- Multifunctional regulation of VAMP3 in exocytic and endocytic pathways of RBL-2H3 cells.Frontiers in immunology · 2022Article
- Receptor/Raft Ratio Is a Determinant for LRP6 Phosphorylation and WNT/β-Catenin Signaling.Frontiers in cell and developmental biology · 2021Article
- Review
- Disrupted Lipid Raft Shuttling of FcεRI by n-3 Polyunsaturated Fatty Acid Is Associated With Ligation of G Protein-Coupled Receptor 120 (GPR120) in Human Mast Cell Line LAD2.Frontiers in nutrition · 2020Article
- Pseudo-allergic reaction caused by Qingkailing injection partiallyToxicology research · 2019Article
- CD45 exclusion- and cross-linking-based receptor signaling together broaden FcεRI reactivity.Science signaling · 2018Article
- The mystery of membrane organization: composition, regulation and roles of lipid rafts.Nature reviews. Molecular cell biology · 2017Review
- Tracing the Origins of IgE, Mast Cells, and Allergies by Studies of Wild Animals.Frontiers in immunology · 2017Review
- Characterization of the Syk-Dependent T Cell Signaling Response to an Altered Peptide.Journal of immunology (Baltimore, Md. : 1950) · 2016Article
- The Continuing Mystery of Lipid Rafts.Journal of molecular biology · 2016Review
- Motility and stem cell properties induced by the epithelial-mesenchymal transition require destabilization of lipid rafts.Oncotarget · 2016Article
- Polyunsaturated Lipids Regulate Membrane Domain Stability by Tuning Membrane Order.Biophysical journal · 2016Article
- Modeling for (physical) biologists: an introduction to the rule-based approach.Physical biology · 2015Review
- An Interaction Library for the FcεRI Signaling Network.Frontiers in immunology · 2014Review
- Rule-based modeling: a computational approach for studying biomolecular site dynamics in cell signaling systems.Wiley interdisciplinary reviews. Systems biology and medicineReview
Corrections and comments
- Erratum issued
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
We present a model of the early events in mast cell signaling mediated by FcεRI where the plasma membrane is composed of many small ordered lipid domains (rafts), surrounded by a non-order region of lipids consisting of the remaining plasma membrane. The model treats the rafts as transient structures that constantly form and breakup, but that maintain a fixed average number per cell. The rafts have a high propensity for harboring Lyn kinase, aggregated, but not unaggregated receptors, and the linker for the activation of T cells (LAT). Phosphatase activity in the rafts is substantially reduced compared to the nonraft region. We use the model to analyze published experiments on the rat basophilic leukemia (RBL)-2H3 cell line that seem to contradict the notion that rafts offer protection. In these experiments IgE was cross-linked with a multivalent antigen and then excess monovalent hapten was added to break-up cross-links. The dephosphorylation of the unaggregated receptor (nonraft associated) and of LAT (raft associated) were then monitored in time and found to decay at similar rates, leading to the conclusion that rafts offer no protection from dephosphorylation. In the model, because the rafts are transient, a protein that is protected while in a raft will be subject to dephosphorylation when the raft breaks up and the protein finds itself in the nonraft region of the membrane. We show that the model is consistent with the receptor and LAT dephosphorylation experiments while still allowing rafts to enhance signaling by providing substantial protection from phosphatases.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.