ArticlePloS one2012
High-throughput analysis of ammonia oxidiser community composition via a novel, amoA-based functional gene array.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Genomic recovery from rare terrestrial microbes enabled by DNA-based GC-fractionation.ISME communications · 2025Article
- The Effects of Quinone Imine, a New Potent Nitrification Inhibitor, Dicyandiamide, and Nitrapyrin on Target and Off-Target Soil Microbiota.Microbiology spectrum · 2022Article
- Blame It on the Metabolite: 3,5-Dichloroaniline Rather than the Parent Compound Is Responsible for the Decreasing Diversity and Function of Soil Microorganisms.Applied and environmental microbiology · 2018Article
- Analysis of Bacterial Communities in Partial Nitritation and Conventional Nitrification Systems for Nitrogen Removal.Scientific reports · 2018Article
- Ammonia-Oxidizing Archaea Show More Distinct Biogeographic Distribution Patterns than Ammonia-Oxidizing Bacteria across the Black Soil Zone of Northeast China.Frontiers in microbiology · 2018Article
- Freshwater Recirculating Aquaculture System Operations Drive Biofilter Bacterial Community Shifts around a Stable Nitrifying Consortium of Ammonia-OxidizingFrontiers in microbiology · 2017Article
- Quantitative and compositional responses of ammonia-oxidizing archaea and bacteria to long-term field fertilization.Scientific reports · 2016Article
- Review
- The large-scale distribution of ammonia oxidizers in paddy soils is driven by soil pH, geographic distance, and climatic factors.Frontiers in microbiology · 2015Article
- Development of a whole community genome amplification-assisted DNA microarray method to detect functional genes involved in the nitrogen cycle.World journal of microbiology & biotechnology · 2014Article
- A microarray for assessing transcription from pelagic marine microbial taxa.The ISME journal · 2014Article
- Application of a novel functional gene microarray to probe the functional ecology of ammonia oxidation in nitrifying activated sludge.PloS one · 2013Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Advances in microbial ecology research are more often than not limited by the capabilities of available methodologies. Aerobic autotrophic nitrification is one of the most important and well studied microbiological processes in terrestrial and aquatic ecosystems. We have developed and validated a microbial diagnostic microarray based on the ammonia-monooxygenase subunit A (amoA) gene, enabling the in-depth analysis of the community structure of bacterial and archaeal ammonia oxidisers. The amoA microarray has been successfully applied to analyse nitrifier diversity in marine, estuarine, soil and wastewater treatment plant environments. The microarray has moderate costs for labour and consumables and enables the analysis of hundreds of environmental DNA or RNA samples per week per person. The array has been thoroughly validated with a range of individual and complex targets (amoA clones and environmental samples, respectively), combined with parallel analysis using traditional sequencing methods. The moderate cost and high throughput of the microarray makes it possible to adequately address broader questions of the ecology of microbial ammonia oxidation requiring high sample numbers and high resolution of the community composition.
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