ArticlePloS one2012
Aging affects the transcriptional regulation of human skeletal muscle disuse atrophy.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 88 papers, 2 of them syntheses that pooled it.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
88 citing papers in PubMed, 2 syntheses or guidelines pooled it, 156 citations in OpenAlex.
- Single-leg disuse decreases skeletal muscle strength, size, and power in uninjured adults: A systematic review and meta-analysis.Journal of cachexia, sarcopenia and muscle · 2023Pooled it
- Effect of Immobilisation on Neuromuscular Function In Vivo in Humans: A Systematic Review.Sports medicine (Auckland, N.Z.) · 2019Pooled it
- Effects of short-term hand immobilization on motor functions across domains in adults.Physiological reports · 2026Trial
- Two-week step-reduction has limited negative effects on physical function and metabolic health in older adults.European journal of applied physiology · 2024Trial
- Muscle protein breakdown is impaired during immobilization compared to during a subsequent retraining period in older men: no effect of anti-inflammatory medication.Pflugers Archiv : European journal of physiology · 2020Trial
- Impact of dairy protein during limb immobilization and recovery on muscle size and protein synthesis; a randomized controlled trial.Journal of applied physiology (Bethesda, Md. : 1985) · 2018Trial
- Neuromuscular Electrical Stimulation Combined with Protein Ingestion Preserves Thigh Muscle Mass But Not Muscle Function in Healthy Older Adults During 5 Days of Bed Rest.Rejuvenation research · 2017Trial
- Ageing is associated with diminished muscle re-growth and myogenic precursor cell expansion early after immobility-induced atrophy in human skeletal muscle.The Journal of physiology · 2013Trial
- Musculoskeletal decay and bone-muscle crosstalk during physical disuse: Mechanisms and integrated countermeasures.Experimental physiology · 2026Review
- Robust immune cell infiltration and macrophage senescence occur within a week of recovery after limb immobilization in older adult skeletal muscle.The Journal of physiology · 2026Article
- Muscle atrophy after ACL reconstruction involves molecular mechanisms beyond unloading.Journal of applied physiology (Bethesda, Md. : 1985) · 2026Article
- Effects of neuromuscular electrical stimulation on voluntary muscle activation and peripheral muscle contractility following short-term bed rest.Experimental physiology · 2025Article
- Changes in motor unit conduction velocity after unilateral lower-limb suspension and active recovery are correlated with muscle ion channel gene expression.Experimental physiology · 2025Article
- Supraspinatus Muscle Regeneration Following Rotator Cuff Tear: A Study of the Biomarkers Pax7, MyoD, and Myogenin.International journal of molecular sciences · 2024Article
- Acute Sarcopenia: Mechanisms and Management.Nutrients · 2024Review
- Profiling muscle transcriptome in mice exposed to microgravity using gene set enrichment analysis.NPJ microgravity · 2024Article
- The Effects of Heat Therapy During Immobilization and Rehabilitation on Muscle Atrophy and Strength Loss at Return to Sports in Healthy Humans.Orthopaedic journal of sports medicine · 2024Article
- Optogenetic induction of mechanical muscle stress identifies myosin regulatory ubiquitin ligase NHL-1 in C. elegans.Nature communications · 2024Article
- Five days of bed rest in young and old adults: Retainment of skeletal muscle mass with neuromuscular electrical stimulation.Physiological reports · 2024Article
- Bed-rest and exercise remobilization: Concurrent adaptations in muscle glucose and protein metabolism.Journal of cachexia, sarcopenia and muscle · 2024Article
28 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
14 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Important insights concerning the molecular basis of skeletal muscle disuse-atrophy and aging related muscle loss have been obtained in cell culture and animal models, but these regulatory signaling pathways have not previously been studied in aging human muscle. In the present study, muscle atrophy was induced by immobilization in healthy old and young individuals to study the time-course and transcriptional factors underlying human skeletal muscle atrophy. The results reveal that irrespectively of age, mRNA expression levels of MuRF-1 and Atrogin-1 increased in the very initial phase (2-4 days) of human disuse-muscle atrophy along with a marked reduction in PGC-1α and PGC-1β (1-4 days) and a ~10% decrease in myofiber size (4 days). Further, an age-specific decrease in Akt and S6 phosphorylation was observed in young muscle within the first days (1-4 days) of immobilization. In contrast, Akt phosphorylation was unchanged in old muscle after 2 days and increased after 4 days of immobilization. Further, an age-specific down-regulation of MuRF-1 and Atrogin-1 expression levels was observed following 2 weeks of immobilization, along with a slowing atrophy response in aged skeletal muscle. Neither the immediate loss of muscle mass, nor the subsequent age-differentiated signaling responses could be explained by changes in inflammatory mediators, apoptosis markers or autophagy indicators. Collectively, these findings indicate that the time-course and regulation of human skeletal muscle atrophy is age dependent, leading to an attenuated loss in aging skeletal muscle when exposed to longer periods of immobility-induced disuse.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.