ArticleJournal of clinical microbiology2013
Detection of the A2058G and A2059G 23S rRNA gene point mutations associated with azithromycin resistance in Treponema pallidum by use of a TaqMan real-time multiplex PCR assay.
Article in Journal of clinical microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers, 1 of them a synthesis that pooled it.
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Who cites it
33 citing papers in PubMed, 1 synthesis or guideline pooled it, 69 citations in OpenAlex.
- On the pathway to better birth outcomes? A systematic review of azithromycin and curable sexually transmitted infections.Expert review of anti-infective therapy · 2013Pooled it
- Comparative efficacy of low-dose versus standard-dose azithromycin for patients with yaws: a randomised non-inferiority trial in Ghana and Papua New Guinea.The Lancet. Global health · 2018Trial
- The Critical Role of Penicillin in Syphilis Treatment and Emerging Resistance Challenges.Diseases (Basel, Switzerland) · 2025Review
- Yaws in Africa: Past, Present and Future.Diseases (Basel, Switzerland) · 2025Review
- Molecular investigation ofMicrobiology spectrum · 2024Article
- Multiplex Loop-Mediated Isothermal Amplification Coupled Lateral Flow Assay for Point-of-Care Detection of Syphilis.Indian journal of microbiology · 2024Article
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- A 2021 Update on Syphilis: Taking Stock from Pathogenesis to Vaccines.Pathogens (Basel, Switzerland) · 2021Review
- Treponema pallidum Macrolide Resistance and Molecular Epidemiology in Southern Africa, 2008 to 2018.Journal of clinical microbiology · 2021Article
- Treponema pallidum in female sex workers from the Brazilian Marajó Archipelago: prevalence, risk factors, drug-resistant mutations and coinfections.Transactions of the Royal Society of Tropical Medicine and Hygiene · 2021Article
- Multiplex Mediator Displacement Loop-Mediated Isothermal Amplification for Detection of Treponema pallidum and Haemophilus ducreyi.Emerging infectious diseases · 2020Article
- Successful isolation of Treponema pallidum strains from patients' cryopreserved ulcer exudate using the rabbit model.PloS one · 2020Article
- Strain diversity of Treponema pallidum subsp. pertenue suggests rare interspecies transmission in African nonhuman primates.Scientific reports · 2019Article
- PCR detection for syphilis diagnosis: Status and prospects.Journal of clinical laboratory analysis · 2019Review
- Advances in the Treatment of Yaws.Tropical medicine and infectious disease · 2018Review
- Gene subtype analysis ofExperimental and therapeutic medicine · 2018Article
- Diagnostics for Yaws Eradication: Insights From Direct Next-Generation Sequencing of Cutaneous Strains of Treponema pallidum.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2018Article
- Community-based mass treatment with azithromycin for the elimination of yaws in Ghana-Results of a pilot study.PLoS neglected tropical diseases · 2018Article
- Syphilis: Re-emergence of an old foe.Microbial cell (Graz, Austria) · 2016Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Macrolide treatment failure in syphilis patients is associated with a single point mutation (either A2058G or A2059G) in both copies of the 23S rRNA gene in Treponema pallidum strains. The conventional method for the detection of both point mutations uses nested PCR combined with restriction enzyme digestions, which is laborious and time-consuming. We initially developed a TaqMan-based real-time duplex PCR assay for detection of the A2058G mutation, and upon discovery of the A2059G mutation, we modified the assay into a triplex format to simultaneously detect both mutations. The point mutations detected by the real-time triplex PCR were confirmed by pyrosequencing. A total of 129 specimens PCR positive for T. pallidum that were obtained from an azithromycin resistance surveillance study conducted in the United States were analyzed. Sixty-six (51.2%) of the 129 samples with the A2058G mutation were identified by both real-time PCR assays. Of the remaining 63 samples that were identified as having a macrolide-susceptible genotype by the duplex PCR assay, 17 (27%) were found to contain the A2059G mutation by the triplex PCR. The proportions of macrolide-susceptible versus -resistant genotypes harboring either the A2058G or the A2059G mutation among the T. pallidum strains were 35.6, 51.2, and 13.2%, respectively. None of the T. pallidum strains examined had both point mutations. The TaqMan-based real-time triplex PCR assay offers an alternative to conventional nested PCR and restriction fragment length polymorphism analyses for the rapid detection of both point mutations associated with macrolide resistance in T. pallidum.
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