ArticleBiomaterials2013
Reconfiguring the architectures of cationic helical polypeptides to control non-viral gene delivery.
Article in Biomaterials, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Synergistic effect of Aloe Vera hydrogels with imatinib for pH-responsive drug release in breast cancer treatment.Drug delivery and translational research · 2026Article
- Structural Engineering of l-Aspartic Amphiphilic Polyesters for Enzyme-Responsive Drug Delivery and Bioimaging in Cancer Cells.ACS polymers Au · 2024Article
- Exploring Advanced CRISPR Delivery Technologies for Therapeutic Genome Editing.Small science · 2024Article
- Techniques for investigating lncRNA transcript functions in neurodevelopment.Molecular psychiatry · 2024Review
- pH and HTheranostics · 2024Article
- Recent Genome-Editing Approaches toward Post-Implanted Fetuses in Mice.Biotech (Basel (Switzerland)) · 2023Review
- Nanoparticle Delivery of CRISPR/Cas9 for Genome Editing.Frontiers in genetics · 2021Review
- Charge-switchable zwitterionic polycarboxybetaine particle as an intestinal permeation enhancer for efficient oral insulin delivery.Theranostics · 2021Article
- Induction of a higher-ordered architecture in glatiramer acetate improves its biological efficiency in an animal model of multiple sclerosis.Biomaterials science · 2020Article
- Modular design and self-assembly of multidomain peptides towards cytocompatible supramolecular cell penetrating nanofibers.RSC advances · 2020Article
- Systemic siRNA delivery to tumors by cell-penetrating α-helical polypeptide-based metastable nanoparticles.Nanoscale · 2018Article
- Stimuli-Responsive Polypeptides for Biomedical Applications.Polymers · 2018Review
- Non-viral gene delivery systems for tissue repair and regeneration.Journal of translational medicine · 2018Review
- Copolymer micelles function as pH-responsive nanocarriers to enhance the cytotoxicity of a HER2 aptamer in HER2-positive breast cancer cells.International journal of nanomedicine · 2018Article
- Overcoming Gene-Delivery Hurdles: Physiological Considerations for Nonviral Vectors.Trends in biotechnology · 2016Review
- Exploring advantages/disadvantages and improvements in overcoming gene delivery barriers of amino acid modified trimethylated chitosan.Pharmaceutical research · 2015Article
- Zwitterionic poly(carboxybetaine)-based cationic liposomes for effective delivery of small interfering RNA therapeutics without accelerated blood clearance phenomenon.Theranostics · 2015Article
- A rationally designed nanoparticle for RNA interference therapy in B-lineage lymphoid malignancies.EBioMedicine · 2014Article
- Trigger-responsive, fast-degradable poly(β-amino ester)s for enhanced DNA unpackaging and reduced toxicity.Biomaterials · 2014Article
- Polypeptides with quaternary phosphonium side chains: synthesis, characterization, and cell-penetrating properties.Biomacromolecules · 2014Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Poly(γ-4-((2-(piperidin-1-yl)ethyl)aminomethyl)benzyl-l-glutamate) (PPABLG), a cationic helical polypeptide, has been recently developed by us as an effective non-viral gene delivery vector. In attempts to elucidate the effect of molecular architecture on the gene delivery efficiencies and thereby identify a potential addition to PPABLG with improved transfection efficiency and reduced cytotoxicity, we synthesized PEG-PPABLG copolymers with diblock, triblock, graft, and star-shaped architectures via a controlled ring-opening polymerization. The PPABLG segment in all copolymers adopted helical structure; all copolymers displayed structure-related cell penetration properties and gene transfection efficiencies. In HeLa and HepG-2 cells, diblock and triblock copolymers exhibited reduced membrane activities and cytotoxicities but uncompromised gene transfection efficiencies compared to the non-PEGylated homo-PPABLG. The graft copolymer revealed lower DNA binding affinity and membrane activity presumably due to the intramolecular entanglement between the grafted PEG segments and charged side chains that led to reduced transfection efficiency. The star copolymer, adopting a spherical architecture with high density of PPABLG, afforded the highest membrane activity and relatively low cytotoxicity, which contributed to its potent gene transfection efficiency that outperformed the non-PEGylated PPABLG and Lipofectamine™ 2000 by 3-5 and 3-134 folds, respectively. These findings provide insights into the molecular design of cationic polymers, especially helical polypeptides towards gene delivery.
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Registered trials
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