ArticleBiophysical journal2012
The molecular mechanism underlying mechanical anisotropy of the protein GB1.
Article in Biophysical journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Engineering an artificial catch bond using mechanical anisotropy.Nature communications · 2024Article
- The transient expression of recombinant proteins in plant cell packs facilitates stable isotope labelling for NMR spectroscopy.Plant biotechnology journal · 2022Article
- Mapping Mechanostable Pulling Geometries of a Therapeutic Anticalin/CTLA-4 Protein Complex.Nano letters · 2022Article
- Contribution of hydrophobic interactions to protein mechanical stability.Computational and structural biotechnology journal · 2022Article
- Anisotropy in mechanical unfolding of protein upon partner-assisted pulling and handle-assisted pulling.Communications biology · 2021Article
- Binding of a Telomestatin Derivative Changes the Mechanical Anisotropy of a Human Telomeric G-Quadruplex.Angewandte Chemie (International ed. in English) · 2019Article
- Effects of ligand binding on the mechanical stability of protein GB1 studied by steered molecular dynamics simulation.Journal of molecular modeling · 2016Article
- Monovalent Strep-Tactin for strong and site-specific tethering in nanospectroscopy.Nature nanotechnology · 2016Article
- Coarse-Grained Simulations of Topology-Dependent Mechanisms of Protein Unfolding and Translocation Mediated by ClpY ATPase Nanomachines.PLoS computational biology · 2016Article
- Mapping Mechanical Force Propagation through Biomolecular Complexes.Nano letters · 2015Article
- Nucleotides regulate the mechanical hierarchy between subdomains of the nucleotide binding domain of the Hsp70 chaperone DnaK.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Polyproteins in structural biology.Current opinion in structural biology · 2015Review
- Force-induced remodelling of proteins and their complexes.Current opinion in structural biology · 2015Review
- Effective harmonic potentials: insights into the internal cooperativity and sequence-specificity of protein dynamics.PLoS computational biology · 2013Article
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5 authors.
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Abstract
Mechanical responses of elastic proteins are crucial for their biological function and nanotechnological use. Loading direction has been identified as one key determinant for the mechanical responses of proteins. However, it is not clear how a change in pulling direction changes the mechanical unfolding mechanism of the protein. Here, we combine protein engineering, single-molecule force spectroscopy, and steered molecular dynamics simulations to systematically investigate the mechanical response of a small globular protein GB1. Force versus extension profiles from both experiments and simulations reveal marked mechanical anisotropy of GB1. Using native contact analysis, we relate the mechanically robust shearing geometry with concurrent rupture of native contacts. This clearly contrasts the sequential rupture observed in simulations for the mechanically labile peeling geometry. Moreover, we identify multiple distinct mechanical unfolding pathways in two loading directions. Implications of such diverse unfolding mechanisms are discussed. Our results may also provide some insights for designing elastomeric proteins with tailored mechanical properties.
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