ArticleBiophysical journal2012
Morphometric image analysis of giant vesicles: a new tool for quantitative thermodynamics studies of phase separation in lipid membranes.
Article in Biophysical journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
11 citing papers in PubMed, 40 citations in OpenAlex.
- Interfacial Organization and Structural Changes in Model Lung Surfactants Induced by Methylxanthines.Langmuir : the ACS journal of surfaces and colloids · 2026Article
- Stability of multilamellar lipid tubules in excess water.European biophysics journal : EBJ · 2023Article
- DisGUVery: A Versatile Open-Source Software for High-Throughput Image Analysis of Giant Unilamellar Vesicles.ACS synthetic biology · 2023Article
- Recent Advances in Light Energy Conversion with Biomimetic Vesicle Membranes.Chembiochem : a European journal of chemical biology · 2021Review
- Giant Unilamellar Vesicle Electroformation: What to Use, What to Avoid, and How to Quantify the Results.Membranes · 2021Review
- Principles and Applications of Biological Membrane Organization.Annual review of biophysics · 2020Review
- Effects of artepillin C on model membranes displaying liquid immiscibility.Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica · 2019Article
- Composition Fluctuations in Lipid Bilayers.Biophysical journal · 2017Article
- Capturing suboptical dynamic structures in lipid bilayer patches formed from free-standing giant unilamellar vesicles.Nature protocols · 2017Article
- Phase Composition Control in Microsphere-Supported Biomembrane Systems.Langmuir : the ACS journal of surfaces and colloids · 2017Article
- Minimal effect of lipid charge on membrane miscibility phase behavior in three ternary systems.Biophysical journal · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have developed a strategy to determine lengths and orientations of tie lines in the coexistence region of liquid-ordered and liquid-disordered phases of cholesterol containing ternary lipid mixtures. The method combines confocal-fluorescence-microscopy image stacks of giant unilamellar vesicles (GUVs), a dedicated 3D-image analysis, and a quantitative analysis based in equilibrium thermodynamic considerations. This approach was tested in GUVs composed of 1,2-dioleoyl-sn-glycero-3-phosphocholine/1,2-palmitoyl-sn-glycero-3-phosphocholine/cholesterol. In general, our results show a reasonable agreement with previously reported data obtained by other methods. For example, our computed tie lines were found to be nonhorizontal, indicating a difference in cholesterol content in the coexisting phases. This new, to our knowledge, analytical strategy offers a way to further exploit fluorescence-microscopy experiments in GUVs, particularly retrieving quantitative data for the construction of three lipid-component-phase diagrams containing cholesterol.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.