ArticleBiophysical journal2012
Coiled-coil formation on lipid bilayers--implications for docking and fusion efficiency.
Article in Biophysical journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 32 citations in OpenAlex.
- SNARE Modulators and SNARE Mimetic Peptides.Biomolecules · 2022Review
- The Coiled-Coil Forming Peptide (KVSALKE)Advanced healthcare materials · 2022Article
- Membrane fusion studied by colloidal probes.European biophysics journal : EBJ · 2021Review
- Interaction of SNARE Mimetic Peptides with Lipid bilayers: Effects of Secondary Structure, Bilayer Composition and Lipid Anchoring.Scientific reports · 2019Article
- Influence of Membrane-Fusogen Distance on the Secondary Structure of Fusogenic Coiled Coil Peptides.Langmuir : the ACS journal of surfaces and colloids · 2019Article
- Cholesterol enhances influenza binding avidity by controlling nanoscale receptor clustering.Chemical science · 2018Article
- Artificial Membrane Fusion Triggered by Strain-Promoted Alkyne-Azide Cycloaddition.Bioconjugate chemistry · 2017Article
- Drug Delivery via Cell Membrane Fusion Using Lipopeptide Modified Liposomes.ACS central science · 2016Article
- Disentangling Viral Membrane Fusion from Receptor Binding Using Synthetic DNA-Lipid Conjugates.Biophysical journal · 2016Article
- Geometry of the Contact Zone between Fused Membrane-Coated Beads Mimicking Cell-Cell Fusion.Biophysical journal · 2016Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Coiled-coil formation of four different oligopeptides was characterized in solution, on hydrogels, and on membranes by employing circular dichroism spectroscopy, surface plasmon resonance spectroscopy, attenuated total reflection infrared spectroscopy, and ellipsometry. Peptide sequences rich in either glutamic acid (E: E3Cys, i-E3Cys) or lysine (K: K3Cys, i-K3Cys) were used to represent minimal mimics of eukaryotic SNARE motifs. Half of the peptides were synthesized in reverse sequence, so that parallel and antiparallel heptad coiled-coil structures were formed. Either E-peptides or K-peptides were attached covalently to phospholipid anchors via maleimide chemistry, and served as receptors for the recognition of the corresponding binding partners added to solution. Attenuated total reflection infrared spectroscopy of single bilayers confirmed the formation of coiled-coil complexes at the membrane interface. Coiled-coil formation in solution, as compared with association at the membrane surface, displays considerably larger binding constants that are largely attributed to loss of translational entropy at the interface. Finally, the fusogenicity of the various coiled-coil motifs was explored, and the results provide clear evidence that hemifusion followed by full fusion requires a parallel orientation of α-helices, whereas antiparallel oriented coiled-coil motifs display only docking.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.