ArticleGlycobiology2013
Elucidation of a novel lipid A α-(1,1)-GalA transferase gene (rgtF) from Mesorhizobium loti: Heterologous expression of rgtF causes Rhizobium etli to synthesize lipid A with α-(1,1)-GalA.
Article in Glycobiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 20 citations in OpenAlex.
- Article
- Rhizobium determinants of rhizosphere persistence and root colonization.The ISME journal · 2024Article
- Caulobacter lipid A is conditionally dispensable in the absence of fur and in the presence of anionic sphingolipids.Cell reports · 2022Article
- Recently EvolvedFrontiers in cellular and infection microbiology · 2022Article
- Article
- Alpha-Gal Syndrome: Involvement ofFrontiers in cellular and infection microbiology · 2021Article
- Tick Saliva and the Alpha-Gal Syndrome: Finding a Needle in a Haystack.Frontiers in cellular and infection microbiology · 2021Review
- The Lipid A from the Lipopolysaccharide of the Phototrophic BacteriumInternational journal of molecular sciences · 2020Article
- Lipid A fromInternational journal of molecular sciences · 2020Article
- Studies on lipid A isolated from Phyllobacterium trifolii PETP02Antonie van Leeuwenhoek · 2017Article
- Growth and Survival of Mesorhizobium loti Inside Acanthamoeba Enhanced Its Ability to Develop More Nodules on Lotus corniculatus.Microbial ecology · 2015Article
- Occurrence of an unusual hopanoid-containing lipid A among lipopolysaccharides from Bradyrhizobium species.The Journal of biological chemistry · 2014Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
An unusual α-(1,1)-galacturonic acid (GalA) lipid A modification has been reported in the lipopolysaccharide of a number of interesting Gram-negative bacteria, including the nitrogen-fixing bacteria Azospirillum lipoferum, Mesorhizobium huakuii and M. loti, the stalk-forming bacterium Caulobacter crescentus and the hyperthermophilic bacterium Aquifex aeolicus. However, the α-(1,1)-GalA transferase (GalAT) gene, which we have named RgtF, was not identified. Species of the Rhizobium genera produce lipid A with α-(1,4')-GalA but not α-(1,1)-GalA. The Rhizobium GalAT, RgtD, is the lipid A α-(1-4')-GalAT which utilizes the lipid donor dodecaprenyl-phosphate GalA (Dod-P-GalA) for GalA transfer. An additional Rhizobium GalAT, RgtE, is required for the biosynthesis of Dod-P-GalA. We predicted candidate rgtF genes in bacterial species known to produce lipid A with α-(1,1)-GalA. In order to determine the predicted rgtF gene function, we cloned the M. loti rgtF gene into an expression plasmid and introduced that plasmid into Rhizobium etli strains that do not contain the rgtF gene nor produce lipid A α-(1,1)-GalA. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis combined with NMR studies revealed that the lipid As from these rgtF-complemented strains were modified with an additional α-(1,1)-GalA attached to the proximal glucosamine.
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