ArticleGenesis (New York, N.Y. : 2000)2013
Development of translating ribosome affinity purification for zebrafish.
Article in Genesis (New York, N.Y. : 2000), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
31 citing papers in PubMed, 42 citations in OpenAlex.
- Analyzing the Translatome of Lymphatic and Venous Endothelial Cells In Vivo via Translating Ribosome Affinity Purification (TRAP).Bio-protocol · 2025Article
- In vivo profiling of the endothelium using 'AngioTag' zebrafish.Angiogenesis · 2025Article
- Arabidopsis as a model for translational research.The Plant cell · 2025Review
- Perinuclear damage from nuclear envelope deterioration elicits stress responses that contribute toScience advances · 2024Article
- Znf598-mediated Rps10/eS10 ubiquitination contributes to the ribosome ubiquitination dynamics during zebrafish development.RNA (New York, N.Y.) · 2023Article
- Translating Ribosome Affinity Purification (TRAP) of Cell Type-specific mRNA from Mouse Brain Lysates.Bio-protocol · 2022Article
- Dopamine neuron-specific LRRK2 G2019S effects on gene expression revealed by translatome profiling.Neurobiology of disease · 2021Article
- Gelsolin and dCryAB act downstream of muscle identity genes and contribute to preventing muscle splitting and branching in Drosophila.Scientific reports · 2021Article
- Trap-TRAP, a Versatile Tool for Tissue-Specific Translatomics in Zebrafish.Frontiers in cell and developmental biology · 2021Article
- RPL-4 and RPL-9 ̶Mediated Ribosome Purifications Facilitate the Efficient Analysis of Gene Expression inG3 (Bethesda, Md.) · 2020Article
- Developmental Regulation of Nuclear Factor Erythroid-2 Related Factors (nrfs) by AHR1b in Zebrafish (Danio rerio).Toxicological sciences : an official journal of the Society of Toxicology · 2019Article
- Article
- Noncanonical translation via deadenylated 3' UTRs maintains primordial germ cells.Nature chemical biology · 2018Article
- Translation can affect the antisense activity of RNase H1-dependent oligonucleotides targeting mRNAs.Nucleic acids research · 2018Article
- Transcriptomic Profiling of Zebrafish Hair Cells Using RiboTag.Frontiers in cell and developmental biology · 2018Article
- The Expanding Toolkit of Translating Ribosome Affinity Purification.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2017Article
- Biotagging of Specific Cell Populations in Zebrafish Reveals Gene Regulatory Logic Encoded in the Nuclear Transcriptome.Cell reports · 2017Article
- Simultaneous Transcriptional and Epigenomic Profiling from Specific Cell Types within Heterogeneous Tissues In Vivo.Cell reports · 2017Article
- Hermes Regulates Axon Sorting in the Optic Tract by Post-Trancriptional Regulation of Neuropilin 1.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2016Article
- Identification of sensory hair-cell transcripts by thiouracil-tagging in zebrafish.BMC genomics · 2015Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The regulation of transcription and translation by specific cell types is essential to generate the cellular diversity that typifies complex multicellular organisms. Tagging and purification of ribosomal proteins has been shown to be an innovative and effective means of characterizing the ribosome bound transcriptome of highly specific cell populations in vivo. To test the feasibility of using translating ribosome affinity purification (TRAP) in zebrafish, we have generated both a ubiquitous TRAP line and a melanocyte-specific TRAP line using the native zebrafish rpl10a ribosomal protein. We have demonstrated the capacity to capture mRNA transcripts bound to ribosomes, and confirmed the expected enrichment of melanocyte specific genes and depletion of non-melanocyte genes when expressing the TRAP construct with a cell specific promoter. We have also generated a generic EGFP-rpl10a Tol2 plasmid construct (Tol2-zTRAP) that can be readily modified to target any additional cell populations with characterized promoters in zebrafish.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.