ArticleLiver transplantation : official publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society2013
In utero transplanted human hepatocytes allow postnatal engraftment of human hepatocytes in pigs.
Article in Liver transplantation : official publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 33 citations in OpenAlex.
- Fetal-to-fetal kidney transplantation in utero.Communications biology · 2025Article
- Limited Expansion of Human Hepatocytes in FAH/Tissue engineering. Part A · 2022Article
- Genetically engineered pigs for xenotransplantation: Hopes and challenges.Frontiers in cell and developmental biology · 2022Review
- Experimental and clinical progress of in utero hematopoietic cell transplantation therapy for congenital disorders.Frontiers in pharmacology · 2022Review
- Molecular and genetic regulation of pig pancreatic islet cell development.Development (Cambridge, England) · 2020Article
- Genetically Engineered Pigs to Study Cancer.International journal of molecular sciences · 2020Review
- A Comprehensive Protocol for Laparotomy in Swine to Facilitate Ultrasound-Guided Injection into the Fetal Intraperitoneal Space.Comparative medicine · 2019Article
- Proceedings from the 2018 Canadian Association for the Study of the Liver Single Topic Conference-Decompensated cirrhosis: from clinic to transplant.Canadian liver journal · 2019Review
- Long-Term Hematopoietic Engraftment of Congenic Amniotic Fluid Stem Cells After in Utero Intraperitoneal Transplantation to Immune Competent Mice.Stem cells and development · 2018Article
- In Utero Stem Cell Transplantation: Potential Therapeutic Application for Muscle Diseases.Stem cells international · 2017Review
- Cell therapy in chronic liver disease.Current opinion in gastroenterology · 2016Review
- Engineered Swine Models of Cancer.Frontiers in genetics · 2016Review
- The effect of time and temperature on viability and performance of Langerhans islets separated from Balb/c mouse after death.Advanced biomedical research · 2015Article
- Continuous molecular adsorbent recirculating system treatment in 69 patients listed for liver transplantation.Scandinavian journal of gastroenterology · 2015Article
- Article
- Fumarylacetoacetate hydrolase deficient pigs are a novel large animal model of metabolic liver disease.Stem cell research · 2014Article
- Cellular therapy for liver disease.Mayo Clinic proceedings · 2014Review
- Portocaval shunt for hepatocyte package: challenging application of small intestinal graft in animal models.Organogenesis · 2013Article
- Transplantable liver production plan: "Yamaton"--liver project, Japan.Organogenesis · 2013Article
- In utero hepatocellular transplantation in rats.Clinical & developmental immunology · 2013Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
In utero cell transplantation (IUCT) can lead to the postnatal engraftment of human cells in the xenogeneic recipient. Most reports of IUCT have involved hematopoietic stem cells. It is unknown whether human hepatocytes used for IUCT in fetal pigs will lead to the engraftment of these same cells in the postnatal environment. In this study, fetal pigs received direct liver injections of 1 × 10(7) human hepatocytes in utero and were delivered by cesarean section at term. The piglets received a second direct liver injection of 5 × 10(7) human hepatocytes 1 week after birth. The serum was analyzed for human albumin 2, 4, and 6 weeks after engraftment. Piglet livers were harvested 6 weeks after transplantation and were examined by immunohistochemistry, polymerase chain reaction, and fluorescence in situ hybridization for human-specific sequences. Piglets undergoing IUCT with human hepatocytes that were postnatally engrafted with human hepatocytes showed significant levels of human albumin production in their serum at all postengraftment time points. Human albumin gene expression, the presence of human hepatocytes, and the presence of human beta-2 microglobulin were all confirmed 6 weeks after engraftment. IUCT in fetal pigs with human hepatocytes early in gestation allowed the engraftment of human hepatocytes, which remained viable and functional for weeks after transplantation. IUCT followed by postnatal engraftment may provide a future means for large-scale expansion of human hepatocytes in genetically engineered pigs.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.