ArticleMicrocirculation (New York, N.Y. : 1994)2013
A chronic scheme of cranial window preparation to study pial vascular reactivity in murine cerebral malaria.
Article in Microcirculation (New York, N.Y. : 1994), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
6 citing papers in PubMed.
- Refinement of a chronic cranial window implant in the rat for longitudinal in vivo two-photon fluorescence microscopy of neurovascular function.Scientific reports · 2019Article
- Reversal of cerebrovascular constriction in experimental cerebral malaria by L-arginine.Scientific reports · 2018Article
- Evaluation of cerebrovascular impedance and wave reflection in mouse by ultrasound.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2015Article
- Vascular dysfunction as a target for adjuvant therapy in cerebral malaria.Memorias do Instituto Oswaldo Cruz · 2014Review
- Real-time imaging reveals the dynamics of leukocyte behaviour during experimental cerebral malaria pathogenesis.PLoS pathogens · 2014Article
- Looking through a cranial window: intravital microscopy for in vivo study of cerebral malaria.Virulence · 2013Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
objectiveThe acute implantation of a cranial window for studying cerebroarteriolar reactivity in living animals involves a highly surgically invasive craniotomy procedure at the time of experimentation, which limits its application in severely ill animals such as in the experimental murine model of cerebral malaria (ECM). To overcome this problem, a chronic window implantation scheme was designed and implemented.
methodsA partial craniotomy is first performed by creating a skull bone flap in the healthy mice, which are then left to recover for one to two weeks, followed by infection to induce ECM. Uninfected animals are utilized as control. When cranial superfusion is needed, the bone flap is retracted and window implantation completed by assembling a perfusion chamber for compound delivery to the exposed brain surface. The presurgical step is intended to minimize surgical trauma on the day of experimentation.
resultsChronic preparations in uninfected mice exhibited remarkably improved stability over acute ones by significantly reducing periarteriolar tissue damage and enhancing cerebroarteriolar dilator responses. The chronic scheme was successfully implemented in ECM mice, which unveiled novel preliminary insights into impaired cerebroarteriolar reactivity and eNOS dysfunction.
conclusionThe chronic scheme presents an innovative approach for advancing our mechanistic understanding on cerebrovascular dysfunction in ECM.
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