ArticleCanadian journal of veterinary research = Revue canadienne de recherche veterinaire2012
Optimized protocol for multiplex nested polymerase chain reaction to detect and differentiate Haemophilus parasuis, Streptococcus suis, and Mycoplasma hyorhinis in formalin-fixed, paraffin-embedded tissues from pigs with polyserositis.
Article in Canadian journal of veterinary research = Revue canadienne de recherche veterinaire, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 17 citations in OpenAlex.
- Development of a Triplex qPCR Assay Based on the TaqMan Probe for the Detection ofMicroorganisms · 2024Article
- Retrospective Analysis of the Detection of Pathogens Associated with the Porcine Respiratory Disease Complex in Routine Diagnostic Samples from Austrian Swine Stocks.Veterinary sciences · 2023Article
- Interactions ofPathogens (Basel, Switzerland) · 2023Article
- Review of the speculative role of co-infections in Streptococcus suis-associated diseases in pigs.Veterinary research · 2021Review
- Article
- Detection and genetic characterization of porcine circovirus 3 from aborted fetuses and pigs with respiratory disease in Korea.Journal of veterinary science · 2018Article
- Streptococcus suis infection: an emerging/reemerging challenge of bacterial infectious diseases?Virulence · 2014Review
- Digoxigenin-labeled in situ hybridization for the detection of Streptococcus suis DNA in polyserositis and a comparison with biotinylated in situ hybridization.The Journal of veterinary medical science · 2014Article
- Program of vaccination and antibiotic treatment to control polyserositis caused by Haemophilus parasuis under field conditions.Canadian journal of veterinary research = Revue canadienne de recherche veterinaire · 2013Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An optimized protocol was developed for the simultaneous detection and differentiation of Haemophilus parasuis, Streptococcus suis, and Mycoplasma hyorhinis in formalin-fixed, paraffin-embedded (FFPE) tissues with multiplex nested polymerase chain reaction (PCR). This method also determines the prevalence of these bacteria in pigs with polyserositis. DNA extraction with a combination of a commercial reagent and proteinase K resulted in more frequent detection of the pathogens than DNA extraction with proteinase K alone. Among FFPE tissue samples from 312 cases of polyserositis in which at least 1 bacterial species was detected, multiplex nested PCR detected H. parasuis in 239 (77%), S. suis in 124 (40%), and M. hyorhinis in 40 (13%). The disease was caused by a single pathogen in 224 (72%) of the cases and multiple pathogens in 88 (28%). Among the pigs positive for H. parasuis, S. suis, and M. hyorhinis by multiplex nested PCR, the pathogen was isolated from only 11%, 35%, and 28%, respectively. Therefore, the PCR protocol developed in this study is a useful diagnostic method when samples are negative after isolation methods and even for samples in which only 1 pathogen was isolated.
Indexed as
Identifiers
23277698PMC3384282W82956858What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.