ArticleProceedings of the National Academy of Sciences of the United States of America2013
Fluorescent dopamine tracer resolves individual dopaminergic synapses and their activity in the brain.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
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Who cites it
57 citing papers in PubMed, 109 citations in OpenAlex.
- Protocol for fluorescent false neurotransmitter live imaging of dopamine release dynamics from individual synapses in acute brain slices.STAR protocols · 2026Article
- Multimodal Imaging Reveals Rapid Catecholamine Uptake and Release by Neutrophils.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Advances in Coumarin Fluorescent Probes for Medical Diagnostics: A Review of Recent Developments.Biosensors · 2026Review
- Membrane Merger Dynamics in Exocytosis and Endocytosis.Advances in experimental medicine and biology · 2026Review
- Molecular Design of SERTlight: A Fluorescent Serotonin Probe for Neuronal Labeling in the Brain.Journal of the American Chemical Society · 2024Article
- 5-HT_FAsTR: a versatile, label-free, high-throughput, fluorescence-based microplate assay to quantify serotonin transport and release.Scientific reports · 2024Article
- Interspecies chimerism with human embryonic stem cells generates functional human dopamine neurons at low efficiency.Stem cell reports · 2024Article
- Illuminating the monoamine transporters: Fluorescently labelled ligands to study dopamine, serotonin and norepinephrine transporters.Basic & clinical pharmacology & toxicology · 2023Review
- Protein aggregation and calcium dysregulation are hallmarks of familial Parkinson's disease in midbrain dopaminergic neurons.NPJ Parkinson's disease · 2022Article
- Role of Protein Damage Inflicted by Dopamine Metabolites in Parkinson's Disease: Evidence, Tools, and Outlook.Chemical research in toxicology · 2022Review
- Letting the little light of mind shine: Advances and future directions in neurochemical detection.Neuroscience research · 2022Review
- Chemical Targeting of Rhodol Voltage-Sensitive Dyes to Dopaminergic Neurons.ACS chemical neuroscience · 2022Article
- Stable expression of the human dopamine transporter in N27 cells as an in vitro model for dopamine cell trafficking and metabolism.Toxicology in vitro : an international journal published in association with BIBRA · 2021Article
- The chemical tools for imaging dopamine release.Cell chemical biology · 2021Review
- Assessing Vesicular Monoamine Transport and Toxicity Using Fluorescent False Neurotransmitters.Chemical research in toxicology · 2021Article
- Neuronal activity modulates alpha-synuclein aggregation and spreading in organotypic brain slice cultures and in vivo.Acta neuropathologica · 2020Article
- Chemical Targeting of Voltage Sensitive Dyes to Specific Cells and Molecules in the Brain.Journal of the American Chemical Society · 2020Article
- New optical methods for detecting monoamine neuromodulators.Current opinion in biomedical engineering · 2019Article
- Selective catecholamine detection in living cells by a copper-mediated oxidative bond cleavage.Chemical science · 2019Article
- The next generation of approaches to investigate the link between synaptic plasticity and learning.Nature neuroscience · 2019Review
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
We recently introduced fluorescent false neurotransmitters (FFNs) as optical tracers that enable the visualization of neurotransmitter release at individual presynaptic terminals. Here, we describe a pH-responsive FFN probe, FFN102, which as a polar dopamine transporter substrate selectively labels dopamine cell bodies and dendrites in ventral midbrain and dopaminergic synaptic terminals in dorsal striatum. FFN102 exhibits greater fluorescence emission in neutral than acidic environments, and thus affords a means to optically measure evoked release of synaptic vesicle content into the extracellular space. Simultaneously, FFN102 allows the measurement of individual synaptic terminal activity by following fluorescence loss upon stimulation. Thus, FFN102 enables not only the identification of dopamine cells and their processes in brain tissue, but also the optical measurement of functional parameters including dopamine transporter activity and dopamine release at the level of individual synapses. As such, the development of FFN102 demonstrates that, by bringing together organic chemistry and neuroscience, molecular entities can be generated that match the endogenous transmitters in selectivity and distribution, allowing for the study of both the microanatomy and functional plasticity of the normal and diseased nervous system.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.