ArticleThe Plant cell2012
A dominant point mutation in a RINGv E3 ubiquitin ligase homoeologous gene leads to cleistogamy in Brassica napus.
Article in The Plant cell, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 27 citations in OpenAlex.
- Article
- Cuticle development and the underlying transcriptome-metabolome associations during early seedling establishment.Journal of experimental botany · 2024Article
- Engineered Cleistogamy inHorticulture research · 2023Article
- A small chromosomal inversion mediated by MITE transposons confers cleistogamy in Brassica napus.Plant physiology · 2022Article
- Genome-Wide Characterization of Serine/Arginine-Rich Gene Family and Its Genetic Effects on Agronomic Traits ofFrontiers in plant science · 2022Article
- Molecular and Functional Analysis of U-box E3 Ubiquitin Ligase Gene Family in Rice (International journal of molecular sciences · 2021Article
- Genome-wide analysis of the auxin/indoleacetic acid (Aux/IAA) gene family in allotetraploid rapeseed (Brassica napus L.).BMC plant biology · 2017Article
- Genome-wide identification, evolution and expression analysis of RING finger protein genes in Brassica rapa.Scientific reports · 2017Article
- Induced Mutagenesis inFrontiers in plant science · 2017Article
- Transcriptome Analysis of a Female-sterile Mutant (Frontiers in plant science · 2017Article
- Article
- Integrated mapping and characterization of the gene underlying the okra leaf trait in Gossypium hirsutum L.Journal of experimental botany · 2016Article
- Ubiquitin-dependent protein degradation at the yeast endoplasmic reticulum and nuclear envelope.Critical reviews in biochemistry and molecular biologyReview
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the allopolyploid Brassica napus, we obtained a petal-closed flower mutation by ethyl methanesulfonate mutagenesis. Here, we report cloning and characterization of the Bn-CLG1A (CLG for cleistogamy) gene and the Bn-clg1A-1D mutant allele responsible for the cleistogamy phenotype. Bn-CLG1A encodes a RINGv E3 ubiquitin ligase that is highly conserved across eukaryotes. In the Bn-clg1A-1D mutant allele, a C-to-T transition converts a Pro at position 325 to a Leu (P325L), causing a dominant mutation leading to cleistogamy. B. napus and Arabidopsis thaliana plants transformed with a Bn-clg1A-1D allele show cleistogamous flowers, and characterization of these flowers suggests that the Bn-clg1A-1D mutation causes a pronounced negative regulation of cutin biosynthesis or loading and affects elongation or differentiation of petal and sepal cells. This results in an inhibition or a delay of petal development, leading to folded petals. A homoeologous gene (Bn-CLG1C), which shows 99.5% amino acid identity and is also constitutively and equally expressed to the wild-type Bn-CLG1A gene, was also identified. We showed that P325L is not a loss-of-function mutation and did not affect expression of Bn-clg1A-1D or Bn-CLG1C. Our findings suggest that P325L is a gain-of-function semidominant mutation, which led to either hyper- or neofunctionalization of a redundant homoeologous gene.
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