ArticleBacteriophage2012
Development of a high throughput assay for indirectly measuring phage growth using the OmniLog(TM) system.
Article in Bacteriophage, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
51 citing papers in PubMed, 89 citations in OpenAlex.
- Systematic combinatorial optimization of three-phage cocktails against multidrug-resistantMicrobiology spectrum · 2026Article
- Broth Optical Density-Based Assessment for Phage Therapy: Turbidity Reduction, Antibacterial Virulence, and Time-Kill.Viruses · 2026Review
- Optical Density-Based Methods in Phage Biology: Titering, Lysis Timing, Host Range, and Phage-Resistance Evolution.Viruses · 2025Review
- Quantifying phage infectivity from characteristics of bacterial population dynamics.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Bacteriophage therapy for multidrug-resistant infections: current technologies and therapeutic approaches.The Journal of clinical investigation · 2025Review
- Review
- ColorPhAST: a visual rapid colorimetric assay for detecting phage-susceptibility inFrontiers in microbiology · 2025Article
- Evaluating phage lytic activity: from plaque assays to single-cell technologies.Frontiers in microbiology · 2025Review
- Translational research priorities for bacteriophage therapeutics.Essays in biochemistry · 2024Review
- Challenges and opportunities of phage therapy forApplied and environmental microbiology · 2024Review
- High throughput platform technology for rapid target identification in personalized phage therapy.Nature communications · 2024Article
- Article
- Phage-layer interferometry: a companion diagnostic for phage therapy and a bacterial testing platform.Scientific reports · 2024Article
- Meeting Report of the Second Symposium of the Belgian Society for Viruses of Microbes and Launch of the Phage Valley.Viruses · 2024Article
- PB-LKS: a python package for predicting phage-bacteria interaction through local K-mer strategy.Briefings in bioinformatics · 2024Article
- A comparison of phage susceptibility testing with two liquid high-throughput methods.Frontiers in microbiology · 2024Article
- Interlaboratory comparison ofJournal of clinical microbiology · 2023Article
- Exploring bacteriophage therapy for drug-resistant bacterial infections.Topics in antiviral medicine · 2023Article
- Review
- Monitoring phage-induced lysis of gram-negatives in real time using a fluorescent DNA dye.Scientific reports · 2023Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 6 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The conventional and most accepted method of measuring the lytic activity of a phage against its bacterial host is the plaque assay. This method is laborious, time consuming and expensive, especially in high throughput analyses where multiple phage-bacterial interactions are required to be monitored simultaneously. It can also vary considerably with the experimenter and by the growth and plating conditions. Alternatively, the lytic activity can be measured indirectly by following the decrease in optical density of the bacterial cultures owing to lysis. Here we describe an automated, high throughput, indirect liquid lysis assay to evaluate phage growth using the OmniLog(TM) system. The OmniLog(TM) system uses redox chemistry, employing cell respiration as a universal reporter. During active growth of bacteria, cellular respiration reduces a tetrazolium dye and produces a color change that is measured in an automated fashion. On the other hand, successful phage infection and subsequent growth of the phage in its host bacterium results in reduced bacterial growth and respiration and a concomitant reduction in color. Here we show that microtiter plate wells inoculated with Bacillus anthracis and phage show decreased or no growth, compared with the wells containing bacteria only or phage resistant bacteria plus phage. Also, we show differences in the kinetics of bacterial growth and the timing of appearance of phage resistant bacteria in the presence of individual phages or a cocktail of B. anthracis specific phages. The results of these experiments indicate that the OmniLog(TM) system could be used reliably for indirectly measuring phage growth in high throughput host range and phage and antibiotics combination studies.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.