ArticleNucleic acids research2013
Circularized synthetic oligodeoxynucleotides serve as promoterless RNA polymerase III templates for small RNA generation in human cells.
Article in Nucleic acids research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 26 citations in OpenAlex.
- Direct dsRNA preparation by promoter-free RCT and RNase H cleavage using one circular dsDNA template with a mismatched bubble.RNA (New York, N.Y.) · 2023Article
- Starting and stopping RNA polymerase III transcription on single-stranded DNA oligonucleotides.RNA (New York, N.Y.) · 2022Article
- RNA polymerase III and antiviral innate immune response.Transcription · 2021Review
- A mutation in POLR3E impairs antiviral immune response and RNA polymerase III.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Transcription polymerase-catalyzed emergence of novel RNA replicons.Science (New York, N.Y.) · 2020Article
- Small extrachromosomal circular DNAs, microDNA, produce short regulatory RNAs that suppress gene expression independent of canonical promoters.Nucleic acids research · 2019Article
- Small RNA-seq: The RNA 5'-end adapter ligation problem and how to circumvent it.Journal of biological methods · 2019Article
- Molecular characterization of cell-free eccDNAs in human plasma.Scientific reports · 2017Article
- Article
- Biological Processes Discovered by High-Throughput Sequencing.The American journal of pathology · 2016Review
- Periodic-shRNA molecules are capable of gene silencing, cytotoxicity and innate immune activation in cancer cells.Nucleic acids research · 2016Article
- Adenylylation of small RNA sequencing adapters using the TS2126 RNA ligase I.RNA (New York, N.Y.) · 2016Article
- Preparation of Small RNAs Using Rolling Circle Transcription and Site-Specific RNA Disconnection.Molecular therapy. Nucleic acids · 2015Article
- New insights into the promoterless transcription of DNA coligo templates by RNA polymerase III.Transcription · 2014Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Synthetic RNA formulations and viral vectors are the two main approaches for delivering small therapeutic RNA to human cells. Here we report findings supporting an alternative strategy in which an endogenous human RNA polymerase (RNAP) is harnessed to make RNA hairpin-containing small RNA from synthetic single-stranded DNA oligonucleotides. We report that circularizing a DNA template strand encoding a pre-microRNA hairpin mimic can trigger its circumtranscription by human RNAP III in vitro and in human cells. Sequence and secondary structure preferences that appear to promote productive transcription are described. The circular topology of the template is required for productive transcription, at least in part, to stabilize the template against exonucleases. In contrast to bacteriophage and Escherichia coli RNAPs, human RNAPs do not carry out rolling circle transcription on circularized templates. While transfected DNA circles distribute between the nucleus and cytosol, their transcripts are found mainly in the cytosol. Circularized oligonucleotides are synthetic, free of the hazards of viral vectors and maintain small RNA information in a stable form that RNAP III can access in a cellular context with, in some cases, near promoter-like precision and biologically relevant efficiency.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.