ArticleNucleic acids research2013
Crystal structure of an intermediate of rotating dimers within the synaptic tetramer of the G-segment invertase.
Article in Nucleic acids research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 31 citations in OpenAlex.
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- Direct observation of subunit rotation during DNA strand exchange by serine recombinases.Nature communications · 2024Article
- Unexplored diversity and ecological functions of transposable phages.The ISME journal · 2023Article
- Structural basis for topological regulation of Tn3 resolvase.Nucleic acids research · 2023Article
- Genome-Wide Identification and Expression Profile Analysis of the SnRK2 Gene Family in Nicotiana tabacum.Biochemical genetics · 2022Article
- Engineering altered protein-DNA recognition specificity.Nucleic acids research · 2018Review
- Snapshots of a molecular swivel in action.Nucleic acids research · 2018Article
- Crossover-site sequence and DNA torsional stress control strand interchanges by the Bxb1 site-specific serine recombinase.Nucleic acids research · 2016Article
- Controlled rotation mechanism of DNA strand exchange by the Hin serine recombinase.Scientific reports · 2016Article
- Controlling tetramer formation, subunit rotation and DNA ligation during Hin-catalyzed DNA inversion.Nucleic acids research · 2015Article
- Serine Resolvases.Microbiology spectrum · 2015Review
- Site-specific DNA Inversion by Serine Recombinases.Microbiology spectrum · 2015Article
- Article
- Exploiting subtle structural differences in heavy-atom derivatives for experimental phasing.Acta crystallographica. Section D, Biological crystallography · 2014Article
- Enhancing the specificity of recombinase-mediated genome engineering through dimer interface redesign.Journal of the American Chemical Society · 2014Article
- Exploiting large non-isomorphous differences for phase determination of a G-segment invertase-DNA complex.Acta crystallographica. Section D, Biological crystallography · 2014Article
- The ins and outs of serine integrase site-specific recombination.Current opinion in structural biology · 2014Review
- Snapshots of a viral RNA polymerase switching gears from transcription initiation to elongation.Virologica Sinica · 2013Review
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The serine family of site-specific DNA recombination enzymes accomplishes strand cleavage, exchange and religation using a synaptic protein tetramer. A double-strand break intermediate in which each protein subunit is covalently linked to the target DNA substrate ensures that the recombination event will not damage the DNA. The previous structure of a tetrameric synaptic complex of γδ resolvase linked to two cleaved DNA strands had suggested a rotational mechanism of recombination in which one dimer rotates 180° about the flat exchange interface for strand exchange. Here, we report the crystal structure of a synaptic tetramer of an unliganded activated mutant (M114V) of the G-segment invertase (Gin) in which one dimer half is rotated by 26° or 154° relative to the other dimer when compared with the dimers in the synaptic complex of γδ resolvase. Modeling shows that this rotational orientation of Gin is not compatible with its being able to bind uncleaved DNA, implying that this structure represents an intermediate in the process of strand exchange. Thus, our structure provides direct evidence for the proposed rotational mechanism of site-specific recombination.
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